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Arabidopsis thaliana

ABSTRACT: Genome-wide identification of nitric-oxide regulated H3K9/14ac sites in Arabidopsis thaliana

PROVIDER: PRJNA323929 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3610900.fastq.gz	Fastqsanger.gz
	SRR3610910.fastq.gz	Fastqsanger.gz
	SRR3610920.fastq.gz	Fastqsanger.gz
	SRR3610930.fastq.gz	Fastqsanger.gz
	SRR3610950.fastq.gz	Fastqsanger.gz

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Genome-wide identification of nitric-oxide regulated H3K9/14ac sites in Arabidopsis thaliana

Project description:We report on the identification of nitric-oxide regulated H3K9/14ac sites in Arabidopsis. For this purpose liquid grown Arabidopsis seedlings were treated with the natural NO-donor S-nitrosoglutathione (GSNO), GSNO in combination with the NO-scavenger cPTIO, GSH (control) or TSA (positive control). Material was crosslinked and harvested 3h and 16h after onset of the treatments. Then, quantitative analysis of the H3K9/14ac patterns across these treatment was performed using DiffBind. By comparison of the GSNO and GSNO/cPTIO treatment, NO-regulated H3K9/14ac sites were identified. These sites were then annotated to the nearest TSS. GO enrichment analysis revealed that NO affects H3K9/14ac at many stress-responsive and photosynthetic genes.

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