Project description:The WP3 strains was cultured at 20°C/0.1 MPa, and then was incubated at high hydrostatic pressure (HHP, 50 MPa) for 120 min. The transcriptional profiles after and before HHP shock were compared.
Project description:The WP3 strains was cultured at 20°C/0.1 MPa, and then was incubated at high hydrostatic pressure (HHP, 50 MPa) for 30 min. The transcriptional profiles after and before HHP shock were compared.
Project description:The DNA binding domain of crp gene was deleted from WP3 genome to construct the WP3ΔcrpD2 strain. The two strains were cultured at 20°C and the transcriptional profiles were compared.
Project description:The cAMP binding domain of crp gene was deleted from WP3 genome to construct the WP3ΔcrpD1 strain. The two strains were cultured at 20°C and the transcriptional profiles were compared.
Project description:The prophage and RF DNA of SW1 were removed from WP3 to construct the phage-free strain. The two strains were cultured at 4°C and the transcriptional profiles were compared.