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Differences found in expression of cell surface & ECM components during development of the rainbow trout ovary & testis.

ABSTRACT: A comparison of "maturing" and "prespawn" ovarian and testicular transciptomes was performed to determine the genes that are involved in regulating gametic and accessory cell function during maturation and development of the rainbow trout gonad. To identify some fo the genes involved in these processee, total RNA was compared between three-year-old normal vs two-year-old normal (maturing) and three-year-old normal vs two-year-old precocious (prespawn) gonadal tissue.

ORGANISM(S): Salmo salar Osmerus mordax Oncorhynchus tshawytscha Coregonus clupeaformis Oncorhynchus mykiss

PROVIDER: GSE8309 | GEO | 2007/06/29

SECONDARY ACCESSION(S): PRJNA101281

REPOSITORIES: GEO

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Project description:The ascending aorta is a common site for age-related aortic diseases. Therefore, age-related changes in the cellular composition and development in the normal human ascending aorta tissue is associated with the pathogenesis of aortic diseases. In this study, we performed single-cell transcriptome sequencing of the normal ascending aorta tissues from 6 organ donors of different ages, namely, the young group (3 months, 1 year, 3 years, and 12 years) and the old group (57 years and 58 years), and evaluated the changes in the composition of the cell types, cellular phenotypes, and the cell-cell communication. The single-cell sequencing data analysis of 65,470 cells from the 6 donor aortic wall specimens identified ten cell types (27 high-resolution subtypes of cells), including endothelial cells, smooth muscle cells, fibroblasts, mesenchymal cells, mesenchymal-fibroblast cells, macrophages, Schwann cells, T cells, natural killer cells, and B cells. In comparison with the young group, the aortic wall tissue of the old group showed significant reduction in the proportion of endothelial cells (young vs. old, 22.47% vs. 10.37%), smooth muscle cells (20.40% vs. 18.50%), fibroblasts (30.21% vs. 13.29%), and mesenchymal cells (5.12% vs 1.55%) and significant increase in the proportion of macrophages (10.94% vs. 17.02%) and T cells (3.80% vs. 26.01%). Functional enrichment analysis of the ligand-receptor pairs between the macrophages and the T cells demonstrated enrichment of pathways related to inflammation and endocrine resistance in the elderly group. Immunohistochemical analysis of the aortic tissue from the 58-year-old donor showed higher expression of the M2 macrophage marker, CD163, in comparison with the aortic tissue of the 12-year-old donor. This confirmed the results of the single-cell sequencing analysis. In summary, single-cell sequencing analysis of the normal ascending aorta tissues from multiple donors of different ages demonstrated significant age-related changes in the proportions of multiple cell types, cellular phenotypes, and cell-cell communications. These age-related changes are likely involved in the pathogenesis of age-related aortic diseases.

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Differences found in expression of cell surface & ECM components during development of the rainbow trout ovary & testis.

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