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Comparison of mRNA transcriptomes reveals differential regulation at four different ages in Sika Deer testes

ABSTRACT: To elucidate the complex physiological process of testis development and spermatogenesis in Sika deer, this study evaluated the changes of mRNA profiles in the four developmental stages of testis the juvenile (1-year-old), adolescence (3-year-old), adult (5-year-old) and aged (10-year-old) stages. The results showed that total total of 23558 differentially expressed (DE) unigenes were obtained. 14918 (8413 up and 6505 down), 4988 (2453 up and 2535 down), 5681 (2929 up and 2752 down) DE unigenes were identified in 3-vs.1-, 5-vs.3- and 10-vs.5-year-old testes, respectively. By integrating mRNA expression profiles, we predicted 10790 mRNA-mRNA interaction sites. The target genes were enriched by GO and KEGG pathways to obtain DE mRNA (ALKBH5, Piwil, HIF1A, BRDT, etc.), which play an important role in testis development and spermatogenesis. The data show that DE mRNAs could regulate testis developmental and spermatogenesis through signaling pathways, including MAPK signaling pathway, p53 signaling pathway, PI3K-Akt signaling pathway, Hippo signaling pathway, etc. It provides a useful resource for future studies on the role of genes regulation in testis development and spermatogenesis.

ORGANISM(S): Cervus nippon

PROVIDER: GSE188368 | GEO | 2021/11/10

REPOSITORIES: GEO

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