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The FtsH3 protease is an epistatic master regulator of the iron and phosphate stress responses in the cyanobacterium Synechocystis sp. PCC 6803

ABSTRACT: Regulation of gene expression is a sophisticated process leading to the activation or suppression of genes due to adaptation to environmental stimuli. The membrane-embedded FtsH proteases conserved in bacteria, chloroplasts and mitochondria, are involved in such regulation. The genome of the cyanobacterium Synechocystis sp. PCC 6803 encodes four FtsH homologues FtsH1-4, functioning in the form of oligomeric complexes. Homologue FtsH3 is bound in two hetero-oligomeric complexes, FtsH1/FstH3 and/or FtsH2/FtsH3, respectively. Previous data showed that the FtsH1/FtsH3 complex is involved in the acclimation of cells to iron deficiency by controlling the availability of the transcriptional regulator Fur (Sll0567). To gain more comprehensive insight into the physiological role of FtsH hetero-complexes, we carried out genome-wide expression profiling of a mutant conditionally depleted in FtsH3, grown under nutrient sufficiency and iron depletion. Our results show, that besides Fur, also the SufR and Pho regulons belong to the set of genes controlled by FtsH. Moreover, by combining the transcriptome data with in silico prediction we identified novel targets of Fur in Synechocystis PCC 6803. Fur tends to evoke mostly repression, but also appears to activate some target genes.

ORGANISM(S): Synechocystis sp. PCC 6803

PROVIDER: GSE83303 | GEO | 2016/06/14

SECONDARY ACCESSION(S): PRJNA325523

REPOSITORIES: GEO

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Project description:Iron is an essential cofactor in many metabolic reactions. Mechanisms controlling iron homeostasis need to respond to changes in extracellular conditions, but must also keep the concentration of intracellular iron under strict control, as free ferrous iron (Fe2+) can lead to the generation of reactive oxygen species. Due to its role as redox carrier in photosynthesis, the iron quota in cyanobacteria is about 10 times higher than in model enterobacteria, but the molecular details how such high quota is tightly regulated have remained obscure. Here, we measured time-resolved gene expression changes after iron depletion in the cyanobacterium Synechocystis sp. PCC 6803 using a comprehensive microarray platform monitoring both protein-coding and non-coding transcripts. In total, 644 protein-coding genes were differentially expressed during the first 72h. Many of these proteins are associated with iron transport, photosynthesis or ATP synthesis. Comparing our data with three previous studies, we identified a core set of 28 genes involved in the iron stress response. Among them were genes encoding proteins important for assimilation of inorganic carbon, suggesting a previously unknown link between the carbon and iron regulatory networks. Nine of the 28 genes are of unknown function and constitute key targets for detailed functional analysis. Applying identical statistical and clustering criteria as for the protein-coding fraction, we also identified 10 small RNAs, 62 anti-sense RNAs, four 5M-bM-^@M-^YUTRs and 7 intergenic elements as likely to be involved in the iron regulatory network. Hence, our genome-wide profiling results indicate an unprecedented complexity in the iron-related regulatory network of cyanobacteria. We monitored iron limitation stress induced gene expression changes in the cyanobacterial model organism Synechocystis sp. PCC 6803. We included a control sample without iron-stress (0h) and 5 samples from timepoints after stress induction (3h, 12h, 24h, 48h, 72h). Each timepoint was sampled from two independent biological replicates.

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The FtsH3 protease is an epistatic master regulator of the iron and phosphate stress responses in the cyanobacterium Synechocystis sp. PCC 6803

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