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Transcriptome analysis of satellite cells isolated from an animal model of skeletal muscle hypertrophy

ABSTRACT: Skeletal muscle is a post-mitotic tissue with unique plasticity. It has an extraordinary ability to adjust to changes in its physiological environment by altering its metabolic phenotype and its mass. Satellite cells are the most important myogenic progenitor cells involved in muscle regeneration and remodeling. The activation of these cells is governed by genetic programs involving complex transcriptional responses and can be involved in muscle hypertrophy adaptations. We described here gene expression variations evaluated by microarray analysis in satellite cells isolated from wild type and Magic-F1 transgenic mice, a model of skeletal muscle hypertrophy. The transcriptome analysis shows an altered gene signature in proliferating Magic-F1 satellite cells compared to controls, involving genes associated to muscle growth and vasculogenesis.

ORGANISM(S): Mus musculus

PROVIDER: GSE83771 | GEO | 2016/06/28

SECONDARY ACCESSION(S): PRJNA326953

REPOSITORIES: GEO

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Angiogenesis and Gene Signature in Muscular Hypertrophy

Project description:Adult myogenic progenitor cells (activated satellite cells) express both HGF and its receptor cMet. Following muscle injury, autocrine HGF-Met stimulation plays a key role in promoting activation and early division of satellite cells. Magic-F1 (Met-Activating Genetically Improved Chimeric Factor-1) is an HGF-derived, engineered protein that contains two Met-binding domains that promotes muscle hypertrophy, protecting myogenic precursors against apoptosis, increasing their fusion ability and enhancing muscle differentiation. Hemizygous and homozygous Magic-F1 transgenic mice displayed constitutive muscle hypertrophy. We described here microarray analysis on Magic-F1 myogenic progenitor cells showing an altered gene signatures involving muscle hypertrophy and vasculogenesis compared to wild-type cells. In parallel, we performed a functional analysis on homozygous Magic-F1 transgenic mice versus control, demonstrating that Magic-F1+/+ mice display impairment on running performance. Finally, we show that induced muscle hypertrophy affects positively vascular network, increasing vessel number in fast twitch fibers. This is due to unique features of mammalian skeletal muscle and its remarkable ability to adapt promptly to different physiological demands by changing gene expression profile. Biological triplicates of Magic-F1+/+ and control satellite cells were used for microarray analysis.

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