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ADDITIVE EFFECTS OF MILLIMETER WAVES AND 2 DEOXYGLUCOSE CO-EXPOSURE ON THE HUMAN KERATINOCYTE TRANSCRIPTOME

ABSTRACT: To evaluate the effect of millimeter waves (MMW) exposure on the gene expression, we have employed whole genome microarray expression profiling. Neonatal primary Keratinocyte cells pooled from 3 healthy donors were exposed ex vivo, by 60.4 GHz-MMW, at an incident power density (IPD) of 20 mW/cm² (3 hours of treatment in athermic condition).No modification of keratinocyte transcriptome was observed. Considering that MMW could also impact the cell metabolism, we assessed then effect of glycolysis inhibitor 2 deoxyglucose treatment (2dG, 20 mM during 3 hours) in association with MMW co-exposure. 2dG treatment induces a high modification of the transcriptome (632 coding genes). Genes affected in their expression are linked with transcriptional repression, cellular communication and endoplasmic reticulum homeostasis. The MMW/2dG co-exposition induced a slight modification of cell transcriptome, which reflects the capacity of MMW to interfere with bioenergetic stress response. After RT-PCR validation, 6 genes (SOCS3, SPRY2, TRIB1, FAM46A, CSRNP1 and PPP1R15A) were confirmed as sensitive to MMW exposure during 2dG treatment.

ORGANISM(S): Homo sapiens

PROVIDER: GSE83829 | GEO | 2016/09/20

SECONDARY ACCESSION(S): PRJNA327119

REPOSITORIES: GEO

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Global gene expression analysis reveals importance of thermal effect in cellular response to millimeter wave exposure

Project description:To evaluate the effect of millimeter waves (MMW) exposure on the gene expression, we have employed whole genome microarray expression profiling. Neonatal primary Keratinocyte cells pooled from 3 healthy donors were exposed ex vivo, 3 hours at 20 mW/cmÂ². At this condition, corresponding to the maximum power density authorized for public use, MMW exposure induces a significant increase of the temperature and 665 genes were found differentially expressed. To determine the role of hyperthermia in this response, several controls were performed. When the temperature artificially maintained constant, no specific gene modifications were observed after exposure. However, a heat shock control did not mimic exactly the MMW effect: 34-gene signatures were identified between 20 mW/cm2 MMW exposure and its relative heat shock control. Differential expression of seven genes (ADAMTS6, NOG, IL7R, FADD, JUNB, SNAI2 and HIST1H1A) from this signature were confirmed by duplication of the whole experiment and real-time PCR analysis. Gene expression in human keratinocytes was measured after a 60 GHz-exposure at 20 mW/cm2, for 3 h (Expo). It was compared to cells cultured in unexposed condition (Sham). As millimeter wave (MMW) exposure increase temperature in the cell medium, two controls were performed: 1) MMW exposure (3 h, 60 GHz, 20 mW/cmÂ²) with the heat increase compensated to maintain constant the physiological temperature (CompT_Expo); 2) Heat shock control (HSC) to mimicking the heat effect of MMW exposure.

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