Project description:Background and Methods: High mercury (Hg) levels are a significant concern in many aquatic environments, and adverse effects of Hg exposure in fish can include altered behavior, reproduction, growth, and increased stress responses. In addition, human health concerns over Hg continue to warrant new research on its mechanisms of action. The objectives of this study were to investigate the impacts of Hg on the female largemouth bass (LMB) central nervous system transcriptome by conducting a laboratory injection experiment with 2.5 µg/g body weight in addition to sampling LMB from an environment that contains high levels of Hg (St. Mary’s River, Florida, USA). These animals were compared to LMB that inhibit river systems with lower Hg (Big Wekiva, Santa Fe and St. John’s Rivers Florida, USA). A LMB 8 x 15 K microarray was used for hypothalamic (laboratory) and whole brain (field) transcriptomics analysis. Results: Mercury in the blood of LMB collected from St Mary’s River was significantly elevated 3-fold (~ 0.1 ng Hg/ml blood) compared to LMB collected from the other three sites (~ 0.03 ng Hg/ml blood). In the hypothalamus of Hg injected LMB, there were 937 genes that showed differential expression (raw p = 0.05) but there were no probes that passed an FDR for multiple hypothesis correction. In contrast, in field collected LMB there were 2909 genes that that showed differential expression (raw p = 0.05) of which 38 were significant after correction for multiple hypothesis testing (FDR = 0.05). These included Hsp90 co-chaperone Cdc37, multicopper oxidase, mismatch repair protein Msh6, and 16 kDa heat shock protein A. Functional enrichment in whole brains of LMB collected from the field sites revealed that genes involved in the biological processes of protein folding and targeting, regulation of protein metabolic process, and protein degradation (i.e. ubiquitin cycle) were over-represented. Interestingly, gene set enrichment analysis identifed expression targets that were in common between the laboratory experiment (MeHg injected) and field collected LMB and these consisted of mainly homeobox transcription factors (PROP paired-like homeobox 1, LIM homeobox 3 and paired-like homeodomain 1 and 2). Lastly, prevalent themes emerged in the SNEA expression targets that included neuropeptide receptor signaling, steroid signaling, and structural components, such as beta-actin, integrins, and stress fibres. Conclusions: This study characterizes novel cell signaling pathways that underlie Hg toxicity in the teleostean central nervous system.

Project description:Background and Methods: High mercury (Hg) levels are a significant concern in many aquatic environments, and adverse effects of Hg exposure in fish can include altered behavior, reproduction, growth, and increased stress responses. In addition, human health concerns over Hg continue to warrant new research on its mechanisms of action. The objectives of this study were to investigate the impacts of Hg on the female largemouth bass (LMB) central nervous system transcriptome by conducting a laboratory injection experiment with 2.5 M-BM-5g/g body weight in addition to sampling LMB from an environment that contains high levels of Hg (St. MaryM-bM-^@M-^Ys River, Florida, USA). These animals were compared to LMB that inhibit river systems with lower Hg (Big Wekiva, Santa Fe and St. JohnM-bM-^@M-^Ys Rivers Florida, USA). A LMB 8 x 15 K microarray was used for hypothalamic (laboratory) and whole brain (field) transcriptomics analysis. Results: Mercury in the blood of LMB collected from St MaryM-bM-^@M-^Ys River was significantly elevated 3-fold (~ 0.1 ng Hg/ml blood) compared to LMB collected from the other three sites (~ 0.03 ng Hg/ml blood). In the hypothalamus of Hg injected LMB, there were 937 genes that showed differential expression (raw p = 0.05) but there were no probes that passed an FDR for multiple hypothesis correction. In contrast, in field collected LMB there were 2909 genes that that showed differential expression (raw p = 0.05) of which 38 were significant after correction for multiple hypothesis testing (FDR = 0.05). These included Hsp90 co-chaperone Cdc37, multicopper oxidase, mismatch repair protein Msh6, and 16 kDa heat shock protein A. Functional enrichment in whole brains of LMB collected from the field sites revealed that genes involved in the biological processes of protein folding and targeting, regulation of protein metabolic process, and protein degradation (i.e. ubiquitin cycle) were over-represented. Interestingly, gene set enrichment analysis identifed expression targets that were in common between the laboratory experiment (MeHg injected) and field collected LMB and these consisted of mainly homeobox transcription factors (PROP paired-like homeobox 1, LIM homeobox 3 and paired-like homeodomain 1 and 2). Lastly, prevalent themes emerged in the SNEA expression targets that included neuropeptide receptor signaling, steroid signaling, and structural components, such as beta-actin, integrins, and stress fibres. Conclusions: This study characterizes novel cell signaling pathways that underlie Hg toxicity in the teleostean central nervous system. field study with methyl mercury

Project description:Background and Methods: High mercury (Hg) levels are a significant concern in many aquatic environments, and adverse effects of Hg exposure in fish can include altered behavior, reproduction, growth, and increased stress responses. In addition, human health concerns over Hg continue to warrant new research on its mechanisms of action. The objectives of this study were to investigate the impacts of Hg on the female largemouth bass (LMB) central nervous system transcriptome by conducting a laboratory injection experiment with 2.5 M-BM-5g/g body weight in addition to sampling LMB from an environment that contains high levels of Hg (St. MaryM-bM-^@M-^Ys River, Florida, USA). These animals were compared to LMB that inhibit river systems with lower Hg (Big Wekiva, Santa Fe and St. JohnM-bM-^@M-^Ys Rivers Florida, USA). A LMB 8 x 15 K microarray was used for hypothalamic (laboratory) and whole brain (field) transcriptomics analysis. Results: Mercury in the blood of LMB collected from St MaryM-bM-^@M-^Ys River was significantly elevated 3-fold (~ 0.1 ng Hg/ml blood) compared to LMB collected from the other three sites (~ 0.03 ng Hg/ml blood). In the hypothalamus of Hg injected LMB, there were 937 genes that showed differential expression (raw p = 0.05) but there were no probes that passed an FDR for multiple hypothesis correction. In contrast, in field collected LMB there were 2909 genes that that showed differential expression (raw p = 0.05) of which 38 were significant after correction for multiple hypothesis testing (FDR = 0.05). These included Hsp90 co-chaperone Cdc37, multicopper oxidase, mismatch repair protein Msh6, and 16 kDa heat shock protein A. Functional enrichment in whole brains of LMB collected from the field sites revealed that genes involved in the biological processes of protein folding and targeting, regulation of protein metabolic process, and protein degradation (i.e. ubiquitin cycle) were over-represented. Interestingly, gene set enrichment analysis identifed expression targets that were in common between the laboratory experiment (MeHg injected) and field collected LMB and these consisted of mainly homeobox transcription factors (PROP paired-like homeobox 1, LIM homeobox 3 and paired-like homeodomain 1 and 2). Lastly, prevalent themes emerged in the SNEA expression targets that included neuropeptide receptor signaling, steroid signaling, and structural components, such as beta-actin, integrins, and stress fibres. Conclusions: This study characterizes novel cell signaling pathways that underlie Hg toxicity in the teleostean central nervous system. 4 control and 3 treated hypothalami

Project description:Transcriptional profiling of the digestive gland tissue of female mussel Mytilus galloprovincialis exposed to TCDD, n-TiO2 and their binary mixture Background: Exposure of marine organisms to pollutant mixtures may affect the pattern of contaminant uptake/bioaccumulation, as well as of gene expression in the tissues. Despite the growing concern over the potential biological impact of nanoparticles (NPs) in the aquatic environment, little is known about their interactions with other pollutants.We have recently shown that in the marine mussel Mytilus galloprovincialis exposure to n-TiO2, one of the most widespread type of NPs in use, in combination with 2,3,7,8-TCDD, chosen as model organic xenobiotic, can exert antagonistic or synergistic effects on different biomarkers from the molecular to the tissue level, depending on cell/tissue and type of measured response. An integrated approach involving immunhistochemical and transcriptomic analysis was employed to clarify the itteractive effects of n-TiO2 and TCDD in mussels digestive gland. In particular,TCDD bioaccumulation was evaluated utilizing specific anti-TCDD fluorescent antibodies. Moreover, immunohistochemical evaluation of antioxidant and cytoskeletal components was performed. To provide clues about how the molecular response to the investigated compounds is modulated, we used a cDNA microarray with1673 sequences. In animals exposed only to TiO2, functional genomics analysis of the microarray data (48 differentially expressed genes (DEGs)) highlighted three biological processes, largely dominated by the up-regulation of microtubule-based movement-related genes. Exposure to 2,3,7,8-TCDD yielded 49 DEGs exhibiting distinct patterns in terms of biological processes. Finally, exposure to the mixture rendered 62 GEGs characterized by the regulation of response to chemical stimulus, microtubule-based movement and intracellular signal transduction. Our data should be carefully considered in view of the biological effects of emerging pollutants, particularly in case of mixture chemicals. Transcriptional profiling of the digestive gland tissue of female mussel Mytilus galloprovincialis exposed to TCDD, n-TiO2 and their binary mixture

Project description:Transcriptional profiling of the digestive gland tissue of female mussel Mytilus galloprovincialis exposed to TCDD, n-TiO2 and their binary mixture Background: Exposure of marine organisms to pollutant mixtures may affect the pattern of contaminant uptake/bioaccumulation, as well as of gene expression in the tissues. Despite the growing concern over the potential biological impact of nanoparticles (NPs) in the aquatic environment, little is known about their interactions with other pollutants.We have recently shown that in the marine mussel Mytilus galloprovincialis exposure to n-TiO2, one of the most widespread type of NPs in use, in combination with 2,3,7,8-TCDD, chosen as model organic xenobiotic, can exert antagonistic or synergistic effects on different biomarkers from the molecular to the tissue level, depending on cell/tissue and type of measured response. An integrated approach involving immunhistochemical and transcriptomic analysis was employed to clarify the itteractive effects of n-TiO2 and TCDD in mussels digestive gland. In particular,TCDD bioaccumulation was evaluated utilizing specific anti-TCDD fluorescent antibodies. Moreover, immunohistochemical evaluation of antioxidant and cytoskeletal components was performed. To provide clues about how the molecular response to the investigated compounds is modulated, we used a cDNA microarray with1673 sequences. In animals exposed only to TiO2, functional genomics analysis of the microarray data (48 differentially expressed genes (DEGs)) highlighted three biological processes, largely dominated by the up-regulation of microtubule-based movement-related genes. Exposure to 2,3,7,8-TCDD yielded 49 DEGs exhibiting distinct patterns in terms of biological processes. Finally, exposure to the mixture rendered 62 GEGs characterized by the regulation of response to chemical stimulus, microtubule-based movement and intracellular signal transduction. Our data should be carefully considered in view of the biological effects of emerging pollutants, particularly in case of mixture chemicals.

Dataset Information

Identification of a unique gene expression signature in mercury and 2,3,7,8-tetrachlorodibenzo-p-dioxin co-exposed cells

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