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Expression data from fibroblasts BjhTERT-RhoA-KO and PC3 tumor cells after cocultivation and before

ABSTRACT: Gene expression analysis of BJhTERT RhoA-KO and PC3 mRFP was performed before and after six days confrontation in co-cultivation. Analyses of original and control fibroblasts was also performed. To identify molecules that may mediate the tumor promoting effect of RhoA knock out fibroblasts, we performed gene expression analysis of BJhTERT RhoA-KO and PC3 mRFP cells before the confrontation and then after six days after it, using Affymetrix Whole Transcript Assay platform.

ORGANISM(S): Homo sapiens

PROVIDER: GSE83913 | GEO | 2016/07/01

SECONDARY ACCESSION(S): PRJNA327397

REPOSITORIES: GEO

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Expression data from fibroblasts BjhTERT-RhoA-KO and PC3 tumor cells after cocultivation and before

Project description:Gene expression analysis of BJhTERT RhoA-KO and PC3 mRFP was performed before and after six days confrontation in co-cultivation. Analyses of original and control fibroblasts was also performed. To identify molecules that may mediate the tumor promoting effect of RhoA knock out fibroblasts, we performed gene expression analysis of BJhTERT RhoA-KO and PC3 mRFP cells before the confrontation and then after six days after it, using Affymetrix Whole Transcript Assay platform. Four days old fibroblast monolayer was confronted with PC3 mRFP tumor cells, plated in ratio 1:30 to number of plated fibroblasts. After six days of confrontation cells were sorted by FACS (Fluorescence-activated cell sorting). Total RNA was isolated from each confronted and non-confronted cells with kit. Then 150 ng of total RNA were used for transcriptomic analysis.for RNA extraction and hybridization on Affymetrix microarrays

2016-07-01 | E-GEOD-83913 | biostudies-arrayexpress

Homo sapiens

Project description:Expression data from fibroblasts BjhTERT-RhoA-KO and PC3 tumor cells after cocultivation and before

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RhoA ko BMDC - Control of Homeostasis and Dendritic Cell Survival by the GTPase RhoA

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Increased Rho GTPase activity is associated with decreased tumor inhibitory activity of immortalized fibroblasts

Project description:Clone (Whirly) of human BJhTERT (human foreskin cell line) cells exposed to PC3 mRFP cells Clones (Whirly) of human BJhTERT (human foreskin cell line) cells exposed to PC3 mRFP cells were analyzed with Affymetrix Whole Target (WT) Assay (GeneTitan plate format Gene ST 1.1)

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Project description:Clone (Whirly) of human BJhTERT (human foreskin cell line) cells exposed to PC3 mRFP cells

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Gene expression changes in gastric and colon cancer cell lines after RHOA knockdown.

Project description:RHOA is a recurrently mutated gene in diffuse-type gastric cancer, whose molecular influence in gastric cancer has not been well uncovered. We aimed to clarify the contribution of RHOA to pro-oncogenesis to improve the understanding of the role of RHOA as a therapeutic target. We performed microarray analysis after RHOA knockdown for HGC-27, AGS, CCK-81, and SW948.

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Transcriptomic profiles of CD4+ T lymphocytes from RhoA wild type (WT) and RhoA G17V mice were generated by deep sequencing in duplicate, using Illumina poly-A RNA seq.

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Transcription profiling of human normal and RhoA immortal cells

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2008-10-26 | E-GEOD-12917 | biostudies-arrayexpress

Expression data from RhoA-null and wildtype keratinocytes

Project description:Using mice with a keratinocyte-restricted deletion of the RhoA gene, we explored its role in the regulation of gene expression in vivo and in vitro RNA was isolated from RhoA-null or wildtype keratinocytes, in two different conditions, freshly isolated (in vivo) or cultured (in vitro), using the RNeasy kit (Qiagen, Germany). Hybridization to GeneChip Mouse Genome 430 2.0 Array (Affymetrix, Santa Clara, CA) was carried out at the Copenhagen University Hospital Microarray Center using standard protocols. 2 biological replicates were analyzed for each in vivo genotype (2 samples from RhoA-null, 2 from wildtype), while 3 replicates for each in vitro genotype.

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