Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole transcriptome sequencing of the human thyroid primary cells with knock-down of the FOXE1 gene

ABSTRACT: FOXE1 gene (also known as thyroid transcription factor 2) has been reported to be essential for thyroid gland development and the maintenance of thyroid differentiated status. Current knowledge of FOXE1 regulated genes is mostly based on the expression and functional studies using cell line models. Here we report gene expression profiles of human thyroid primary cells on Day 5 treated with FOXE1 siRNA or nontarget siRNA. The primary cells were generated from fresh nontumorous thyroid tissues obtained from thyroid cancer patients. A number of new dysregulated genes of FOXE1 were discovered, including THBS1 and IGFBP3, which have not been reported as FOXE1 regulated genes before. This study reveals a novel role of FOXE1 in downstream gene regulation and provides a new explanation of FOXE1’s function in thyroid cancer.

ORGANISM(S): Homo sapiens

PROVIDER: GSE83919 | GEO | 2017/02/24

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Whole transcriptome sequencing of the human thyroid primary cells with knock-down of the NRG1 gene

Project description:The protein encoded by the NRG1 gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. Here we report gene expression profiles of human thyroid primary cells on Day 5 treated with NRG1 siRNA. The primary cells were generated from fresh nontumorous thyroid tissues obtained from thyroid cancer patients. A number of new dysregulated genes of NRG1 were discovered. This study reveals a novel role of NRG1 in downstream gene regulation and provides a new explanation of NRG1’s function in thyroid cancer.

2019-06-19 | GSE100322 | GEO

Glis3 regulates the expression of thyroid hormone synthetic genes in coordination with other thyroid transcription factors

Project description:Comparison of cistromes from PAX8, NKX2.1, and FOXE1 ChIP-Seq analysis using mouse thyroid gland and rat thyrocyte PCCl3 cells revealed that there is a significant overlap between GLIS3 binding regions and those of PAX8, NKX2.1, and FOXE1 in genes associated with thyroid hormone biosynthesis.

2023-04-06 | GSE207777 | GEO

Single-cell RNA-Seq of Foxe1KO mouse ESC-derived cells

Project description:Functional thyroid follicles generation in vitro is obtained at high efficiency by doxycycline-mediated expression of Nkx2-1 and Pax8, two proteins involved in early specification of thyroid lineage. Although those factors are sufficient to induce thyroid specification in vitro, other players, such as Foxe1, are also present in early thyroid primordium and its loss-of-function is responsible for thyroid congenital defects in mice and humans. Here we show that Foxe1KO mESCs can be induced to differentiate towards thyroid lineage but the efficiency of thyroid follicular cells generated is drastically low. Moreover, correct expression of maturation genes and capacity to produce thyroid hormone in vitro are completely disrupted. On the other hand, Nkx2-1 expressing cells derived from Foxe1KO have an unexpected ability to deviate to a lung epithelium differentiation program and form organoids containing multiple lung cell types. These findings demonstrate that Foxe1 is required to reinforce thyroid cell fate in vitro and to promote terminal maturation of thyroid follicles.

2023-12-31 | GSE182337 | GEO

Bulk ATAC-seq of Foxe1KO and control Nkx2-1/mKO2+ mouse ESC-derived cells

2023-12-31 | GSE182676 | GEO

Bulk RNA-Seq of Foxe1KO and control Nkx2-1/mKO2+ mouse ESC-derived cells

2023-12-31 | GSE182480 | GEO

Thyroidal gene expression changes in tamoxifen inducible Foxe1 knockout mouse at two weeks post-treatment

Project description:To directly examine the role of Foxe1 in the adult thyroid we generated a model of tamoxifen-inducible Cre mediated Foxe1 deletion in mice of C57BL/6J background (Foxe1flox/flox/Cre). Tamoxifen (0.05g/kg) was administered intraperitoneally to Foxe1flox/flox/Cre mice or Cre control mice aged 6-8 weeks, once daily for five consecutive days. Thyroids were harvested two weeks post-treatment, total RNA extracted and transcriptomic analysis performed using Clariom S Mouse arrays.

2022-10-01 | GSE193441 | GEO

Genome wide profiling of FOXE1 binding sites in PCCL3 rat cells

Project description:FoxE1 transcriptional profiling of rat PCCL3 cells comparing wild type cells with FoxE1 silenced cells and scramble treated with FoxE1 silenced cells. Three-condition experiment, FoxE1 silenced vs. Wild type cells. Biological replicates: 4 replicates, 1pooled sample dye-swap. and FoxE1 silenced vs scramble treated cells. Biological replicates: 4 replicates, 1 pooled sample dye-swap.

2013-05-14 | E-GEOD-42497 | biostudies-arrayexpress

Genome wide profiling of FOXE1 binding sites in PCCL3 rat cells

Project description:FoxE1 transcriptional profiling of rat PCCL3 cells comparing wild type cells with FoxE1 silenced cells and scramble treated with FoxE1 silenced cells.

2013-05-14 | GSE42497 | GEO

Forkhead box E1 (FOXE1) RNAis vs control RNAi in HEKa cells

Project description:Analysis of HEKa cells depleted of Forkhead box E1 (FOXE1). FOXE1 is induced in psoriasis lesions and promotes keratinocytes. Results provide insight into the downstream targets of FOXE1 function in psoriasis.

2021-01-09 | GSE164438 | GEO

Gene profile of human medullary thyroid cancer

Project description:We report gene expression profiling in a series of 17 human medullary thyroid cancer (MTC) tissues, including 8 primary tumors and 9 patient paired neck nodes metastases, in comparison with 3 non-neoplastic thyroid tissues. For the same series we have previously reported miRNA expression profiles (GSE97070 series).

2022-04-27 | GSE196264 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data