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Differentially expressed miRNAs profiling and identification of 2-month-old Irs-1smla/smla mice, Irs-1+/smla mice and Irs-1+/+ mice

ABSTRACT: To investigate the role of insulin-receptor substrate-1 (Irs-1) in the regulation of bone metabolism, we generated Irs-1-deficient mice (Irs1smla/smla). Using the highly sensitive miRNA array, we screened the differentially expressed miRNAs of 2-month-old Irs-1smla/smla mice, Irs-1+/smla mice and Irs-1+/+ mice. Three prediction algorithms (TargetScanS, miRanda, and PicTar) were used to identify the target genes of differentially expressed miRNAs. Furthermore, the quantitative real-time polymerase chain reaction, immunohistochemistry and dual luciferase reporter assay results showed that miR-342-3p is a specific regulator of collagen type I alpha 2 (Col1a2), which will give new insights in disclosing the mechanism of diabetic osteopathy.

ORGANISM(S): Mus musculus

PROVIDER: GSE84310 | GEO | 2016/07/13

SECONDARY ACCESSION(S): PRJNA328801

REPOSITORIES: GEO

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