Project description:To investigate the role of insulin-receptor substrate-1 (Irs-1) in the regulation of bone metabolism, we generated Irs-1-deficient mice (Irs1smla/smla). Using the highly sensitive miRNA array, we screened the differentially expressed miRNAs of 2-month-old Irs-1smla/smla mice, Irs-1+/smla mice and Irs-1+/+ mice. Three prediction algorithms (TargetScanS, miRanda, and PicTar) were used to identify the target genes of differentially expressed miRNAs. Furthermore, the quantitative real-time polymerase chain reaction, immunohistochemistry and dual luciferase reporter assay results showed that miR-342-3p is a specific regulator of collagen type I alpha 2 (Col1a2), which will give new insights in disclosing the mechanism of diabetic osteopathy.
Project description:Identification of differentially expressed genes in young (3 month old) versus aged (24 month old) mouse hematopoietic stem cells. Comparison of genes differentially expressed in hematopoietic stem cells of young mice with conditional deletion of mTOR within vascular endothelium.
Project description:Identification of differentially expressed genes in young (3 month old) versus aged (24 month old) mouse bone marrow derived endothelial cells. Comparison of genes differentially expressed in bone marrow derived endothelial cells of young mice with conditional deletion of mTOR within vascular endothelium.
Project description:MicroRNAs (miRNAs) are small non-coding RNAs that regulate the post transcriptional control of several pathway intermediates, and essential for regulation in skeletal muscle of many species, such as mice, cattle, pig and so on. However, a little number of miRNAs have been reported in the muscle development of goat. In this study, the longissimus dorsi transcripts of goat at 1- and 10-month-old were analyzed for RNA-seq and miRNA-seq. The results showed that 10-month-old Longlin goat expressed 327 up- and 419 down-regulated differentially expressed genes (DEGs) compared with the 1-month-old were founded. In addition, 20 co-up-regulated and 55 co-down-regulated miRNAs involved in muscle fiber hypertrophy of goat were identified in 10-month-old Longlin and Nubian goat compared with 1-month-old. Five miRNA–mRNA pairs (chi-let-7b-3p-MIRLET7A, chi-miR193b-3p-MMP14, chi-miR-355-5p-DGAT2, novel_128-LOC102178119, novel_140-SOD3) involved in the goat skeletal muscle development were identified by miRNA–mRNA negative correlation network analysis. Our results provided an insight into the functional roles of miRNAs of goat muscle-associated miRNAs, allowing us to better understand the transformation of miRNA roles during mammalian muscle development.
Project description:Metabolite profiling was performed on metabolites extracted from the entorhinal cortex and primary visual cortex of 14-15 month old APOE3/3, APOE3/4 and APOE4/4 mice. Metabolites were run on a TOF Mass Spectrometer using an ANP column. Initial analysis was done in an untargeted manner, and processing was done to determine the differentially expressed metabolites based on their mass and retention times. Further analysis was then performed to assign identities to the differentially expressed metabolites using a database of biologically-relevant metabolites whose standards had been run under identical conditions as the samples in the study.
Project description:To investigate the role of miRNAs in the principal cell of kidney collecting duct, a Cre-LoxP recombination strategy was used to generate a mouse model lacking the endoribonuclease Dicer in AQP2 expressing cells (DicerAQP2Cre+). To identify miRNAs altered in DicerAQP2Cre+ mice, small RNA-sequencing analysis was performed on inner medulla tissue from 1-month-old DicerAQP2Cre+ mice and controls (n=3+3 mice). Small RNA-sequencing was used as, relative to probe-based microarrays or RT-qPCR plate arrays, this approach allows both the identification of miRNAs when they have been differentially processed and it offers the possibility to identify novel miRNAs.
Project description:To further analyze the effect of aging and caloric restriction in the microRNA expression, we have employed microarray expression profiling as a discovery platform to identify differentially expressed microRNAs in middle-aged animals and the impact of caloric restriction in the microRNA expression profile. Subcutaneous and visceral adipose tissue were extracted from 3 groups of mice: 3 month-old, 12 month-old fed ad libitum and 12 month-old fed with a caloric restricted diet. Comparisons between young and middle-aged animals in subcutaneous and visceral adipose tissue, and between the 12 month old ad libitum and 12 month old caloric restricted diet in both adipose depots were made.
Project description:To evaluate differentially expressed genes and pathways in hippocampus between young (3-month-old, 3M) and aging (22-month-old, 22M) group. We then performed gene expression profiling analysis using data obtained from mRNA-seq in hippocampus of 3M and 22M group.
Project description:Alzheimer’s disease (AD) is the most common form of dementia with high morbidity in the elderly and the hippocampus is one of the key structures severely affected in AD. We performed hippocampal proteomic analysis of 8-month old App-NLF mice model of AD using a liquid-chromatography/mass-spectrometry system to investigate the proteins that are differentially expressed in order to identify changes in the proteins and molecular pathways that might promote the disease.
