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Cut-and-Run in situ factor profiling maps DNA binding and 3D contact sites at high resolution

ABSTRACT: Unlike Chromatin Immunoprecipitation (ChIP), which fragments and solubilizes total chromatin, Cut-and-Run is performed in situ, allowing for both high-resolution chromatin mapping and probing of the local chromatin environment. When applied to yeast and human nuclei, Cut-and-Run yielded precise transcription factor profiles while avoiding cross-linking and solubilization issues. Cut-and-Run is simple to perform and at low temperatures is inherently robust, with extremely low backgrounds that make it especially cost-effective for transcription factor and chromatin profiling. When used in conjunction with native ChIP-seq and applied to human CTCF, Cut-and-Run mapped high-resolution 3D directional interactions. We conclude that Cut- and-Run is a suitable complement or replacement for ChIP-seq that can also provide 3D mapping information.

ORGANISM(S): Homo sapiens Saccharomyces cerevisiae

PROVIDER: GSE84474 | GEO | 2016/12/30

SECONDARY ACCESSION(S): PRJNA329356

REPOSITORIES: GEO

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