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Response of potato sRNAome to PVYNTN infection [smallRNA-Seq]

ABSTRACT: We aimed at unraveling the role of small RNAs in a complex immune signaling network controlling defense responses that render the plant tolerant to viral infection. We have identified and quantified miRNAs, phasiRNA and virus-derived small RNAs in both, the PVYNTN-tolerant cv. Désirée and its susceptible transgenic counterpart impaired in accumulation of salicylic acid (NahG-Désirée). We have constructed a small RNA regulatory network using in silico prediction results as well as degradome sequencing to link our data to the physiological response (target transcript abundances and hormone levels).

ORGANISM(S): Solanum tuberosum

PROVIDER: GSE84851 | GEO | 2018/02/16

REPOSITORIES: GEO

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Response of potato sRNAome to PVYNTN infection [degradome-Seq]

Project description:We aimed at unraveling the role of small RNAs in a complex immune signaling network controlling defense responses that render the plant tolerant to viral infection. We have identified and quantified miRNAs, phasiRNA and virus-derived small RNAs in both, the PVYNTN-tolerant cv. Désirée and its susceptible transgenic counterpart impaired in accumulation of salicylic acid (NahG-Désirée). We have constructed a small RNA regulatory network using in silico prediction results as well as degradome sequencing to link our data to the physiological response (target transcript abundances and hormone levels).

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MOA:Multiomics analysis of potato response to Potato virus Y (PVY) infection

2019-09-25 | PXD015221 | Pride

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Response of potato sRNAome to PVYNTN infection

Project description:Response of potato sRNAome to PVYNTN infection

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Genome-wide identification of the Phaseolus vulgaris sRNAome using small RNA and degradome sequencing [smallRNA-Seq].

Project description:Background: MiRNAs and phasiRNAs are negative regulators of gene expression. These small RNAs have been extensively studied in plant model species but only 10 mature microRNAs are present in miRBase version 21 and no phasiRNAs have been identified for the legume model Phaseolus vulgaris. Thanks to the recent availability of the first version of the common bean genome, degradome data and small RNA libraries, we are able to present here a catalog of the microRNAs and phasiRNAs of this organism and, particularly, new protagonists of the symbiotic nodulation events. Results: We identified a set of 185 mature miRNAs, including 121 previously unpublished sequences, encoded by 307 precursors and distributed in 98 families. Degradome data allowed us to identify a total of 181 targets for these miRNAs. We reveal two regulatory networks involving conserved miRNAs, known to play crucial roles in the well-establishment of nodules, and novel miRNAs specific of the common bean suggesting a specific action of these sequences. In parallel, we identified 125 loci that potentially produce phased small RNAs and 47 of them present all the characteristics to be triggered by a total of 31 miRNAs, including 14 new miRNAs identified in this study. Conclusions: We provide here a set of new small RNAs, which contribute to the broader scene of the sRNAome of Phaseolus vulgaris. Thanks to the identification of the miRNA targets from degradome analysis and the construction of regulatory networks between the mature microRNAs, we draw up here the probable functional regulation associated with the sRNAome and particularly in N2-fixing symbiotic nodules. Small RNA sequencing from 5 Phaseolus vulgaris tissues

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Genome-wide identification of the Phaseolus vulgaris sRNAome using small RNA and degradome sequencing [smallRNA-Seq].

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miRNAs target conserved motifs of diverse genes encoding NB-LRRs to trigger trans-acting siRNAs in legumes.

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2012-03-29 | E-GEOD-22763 | biostudies-arrayexpress

24-nt phasiRNAs move from tapetal to meiotic cells in maize anthers

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