Dataset Information

MOA:Multiomics analysis of potato response to Potato virus Y (PVY) infection

ABSTRACT: Time series response of potato cv. Désirée, which is tolerant to PVY infection, was analysed in both inoculated as well as upper non-inoculated leaves. Additionally, transgenic plants deficient in accumulation of salicylic acid (NahG- Désirée) were studied in the same setting.

OTHER RELATED OMICS DATASETS IN: PRJNA252955

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Solanum Tuberosum (potato)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Stefane Wienkoop

LAB HEAD: Stefanie Wienkoop

PROVIDER: PXD015221 | Pride | 2019-09-25

REPOSITORIES: Pride

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Publications

Bimodal dynamics of primary metabolism-related responses in tolerant potato-Potato virus Y interaction.

Stare Tjaša T Ramšak Živa Ž Blejec Andrej A Stare Katja K Turnšek Neža N Weckwerth Wolfram W Wienkoop Stefanie S Vodnik Dominik D Gruden Kristina K

BMC genomics 20150919

<h4>Background</h4>Potato virus Y (PVY) is a major pathogen that causes substantial economic losses in worldwide potato production. Different potato cultivars differ in resistance to PVY, from severe susceptibility, through tolerance, to complete resistance. The aim of this study was to better define the mechanisms underlying tolerant responses of potato to infection by the particularly aggressive PVY(NTN) strain. We focused on the dynamics of the primary metabolism-related processes during PVY( ...[more]

PMID: 26386579

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Project description:Several reports have described the involvement of miRNAs in abiotic stresses. However, their role in biotic stress or to beneficial microbes has not been fully explored. In order to understand on the epigenetic regulation in plant in response to nitrogen-fixing bacteria association, we analyzed the sRNA regulation in maize hybrids (Zea mays M-bM-^@M-^S UENF 506-8) inoculated with the beneficial diazotrophic bacteria (Herbaspirillum seropedicae). Deep sequencing analysis was carried out to identify the sRNAs regulated in maize during association with diazotrophic bacteria. For this analysis, maize plants were germinated in wet paper and put in hydroponic system with HoaglandM-bM-^@M-^Ys solution and then inoculated with H. seropedicae for seven days. Mock and inoculated plants were collected and total RNA from a pool of samples was extracted with Trizol reagent. The two sRNA libraries were sequenced by Illumina. The sequences were filtered to remove adaptors and contaminants rRNA and tRNAs, and sequences with 18-28 nt in length were selected. To identify the miRNAs present in these libraries, we used two strategies using the same website (http://srna-tools.cmp.uea.ac.uk): one to identify novel miRNAs using the maize genome (verson 2) and miRCat pipeline; and other to identify conserved miRNAs using the miRBase database (release 13.0, http://microrna.sanger.ac.uk) and miRProf pipeline. We identified 17 novel putative miRNAs candidates and mapped the precursor of these miRNAs in the maize genome. Furthermore, we identified 25 conserved miRNAs families and the differential expressions were analyzed with miRProf pipeline. The bioinformatics analysis of four up-regulated miRNAs (miR397, miR398, miR408 and miR528) in inoculated plant was validated using stemM-bM-^@M-^Sloop RT-PCR assay. Our findings contribute to increase the knowledge of the molecular relation between plants and endophytic bacteria. Screenning of sRNA transcriptome of maize plants inoculated with Herbaspirillum seropedicae after seven days

			Action	DRS
	10_D-WT-MOCK.RAW	Raw
	11_D-WT-MOCK.RAW	Raw
	12_D-WT-MOCK.RAW	Raw
	1_D-NAHG-PVY.RAW	Raw
	1c_D-COI-MOCK.RAW	Raw