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Total RNA: naïve ex vivo mouse OT-1 T lymphocytes, WT vs Lck-/- (OFF)

ABSTRACT: This experiment aims to study the transcriptome of naïve T cells at the steady state. A T cell receptor (TCR) transgenic mouse model (OT-1) posessing a homogenous alpha-beta CD8 T lymphocyte population was used, and cells with intact TCR signalling pathways (WT) were compared to those with defective signal transduction (Lck-/-, OFF). Naïve (CD8b+, CD44low) ex vivo T cells were isolated from the superficial cervicals, axillary, brachial, mesenteric, and inguinal lymph nodes. Total RNA was extracted from the cells, labelled with Cy3, and hybridised onto an Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray.

ORGANISM(S): Mus musculus

PROVIDER: GSE84855 | GEO | 2016/11/30

SECONDARY ACCESSION(S): PRJNA335378

REPOSITORIES: GEO

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Total RNA: 24h activated mouse OT-1 T lymphocytes, WT vs Lck-/- (OFF)

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Polysome profiling: Mouse OT-1 T cells WT vs Lck-/- (OFF)

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2019-11-12 | PXD008258 | Pride

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2024-01-04 | PXD043463 | Pride

The phosphatase DUSP22/JKAP inhibits UBR2-mediated K63-ubiquitination and activation of Lck in TCR signaling

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The phosphatase DUSP22/JKAP inhibits UBR2-mediated K63-ubiquitination and activation of Lck in TCR signaling

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