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Expression profile of bcl11b-deficient NAÏVE CD8+ T cells versus wild type

ABSTRACT: Naïve CD44low CD25low CD8+ T cells from Bcl11bF/F/dLck-iCre and wild type mice at steady state were sorted at 90% purity, RNA was extracted and profile for mRNA expression to identify mRNAs differentially expressed in Bcl11b-/- naïve CD8+ T cells versus wild type. Naïve CD44low CD25low CD8+ T cells purified from Bcl11bF/F/dLck-iCre and wild type mice and investigated for mRNA expression

ORGANISM(S): Mus musculus

SUBMITTER: Dorina Avram

PROVIDER: E-GEOD-56925 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression profile of bcl11b-deficient cd8 T cells during immune response

Project description:Review on the role of Bcl11b in thymus and periphery and impact on immune diseases CD8+ T cells purified from bcl11bf/flckd-cre and wild type mice were infected with Listeria monocytogenes and on day 6 mice were sacrificed and Cd8+ T cells were purified at more than 90% purity. The RNA was purified with trizol and submitted for generation of library and microarrays to establish genes which are regulated in infected KO versus WT cytotoxic T cells.

2014-04-12 | E-GEOD-56713 | biostudies-arrayexpress

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Expression profile of bcl11b-/- preselected DP thymocytes

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2014-04-12 | E-GEOD-56714 | biostudies-arrayexpress

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Project description:expression profile in Bcl11b-deficient Treg cells versus wild type Treg cells Treg cells sorted from Bcl11bF/F/Cd4Cre/Foxp3-GFP+ mice and wild type Foxp3-GFP+ mice Treg cells sorted from Bcl11bF/F/Foxp3Cre mice and wild type mice RNA extracted from sorted Bcl11b-deficient Foxp3-GFP Treg cells form Bcl11bF/F/Cd4Cre/Foxp3-GFP+ mice and wild type Foxp3-GFP Treg cells; expression profile by microarray analysis RNA extracted from sorted Bcl11b-deficient Treg cells form Bcl11bF/F/Foxp3Cre mice and wild type Treg cells; expression profile by microarray analysis

2011-08-27 | E-GEOD-30523 | biostudies-arrayexpress

Tregs from Bcl11b-deficient versus wild type Tregs (CD4Cre and Foxp3Cre systems)

2011-08-28 | GSE30523 | GEO

Transcription factor Bcl11b controls identity and function of mature innate lymphoid cells type II

Project description:Type-2 innate lymphoid cells (ILC2s) promote anti-helminth responses and contribute to allergies. Though Bcl11b has been previously considered a T-lineage identity transcription factor (TF) that restrains the innate-cell genetic programs, we report here that Bcl11b is highly expressed in mature ILC2s and acts upstream of the key ILC2 TFs Gfi1, Gata-3, and of IL-33 receptor IL1rl1 (T1ST2). Additionally, Bcl11b-/- ILC2s de-repressed Rorγt, Ahr and IL-23 receptor, normally expressed in type-3 ILCs (ILC3s). Consequently, Bcl11b-/- ILC2s lost ILC2 functions and gained ILC3 functions, expanding in response to the protease allergen papain, however producing IL-17 and IL-22, and not IL-5 and IL-13, causing lung neutrophilia rather than eosinophilia, and diminished mucus production. Our results broaden Bcl11b's role from a T-cell only TF, and establishes that Bcl11b sustains mature ILC2 genetic and functional programs and lineage fidelity through positive regulation of essential ILC2 TFs and negative regulation of pivotal ILC3 TFs. RNA-seq analysis on sorted ILC2s from the mLNs of Bcl11bF/F Cre-ERT2 and wildtype mice at steady state following tamoxifen mediated deletion of Bcl11b

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Bcl11b depleted and IL-15 stimulated αβ CD8+ T cells acquire a unique innate-like phenotype and are highly functional

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BCL11B

Project description:BCL11B

| PRJNA330816 | ENA

Genome-wide identification of Bcl11b gene targets

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