Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide transcriptional analysis of PTTH-stimulated Bombyx prothoracic gland

ABSTRACT: This is part of a cross-species approach to characterize transcriptional response of the insect prothoracic gland (PG) to the prothoracicotropic hormone (PTTH). Genome-wide response of the PG to PTTH was analyzed in vitro in Bombyx and in vivo in Drosophila, and the results were compared to identify common components in the PTTH signaling pathway.

ORGANISM(S): Bombyx mori

PROVIDER: GSE84963 | GEO | 2016/07/29

SECONDARY ACCESSION(S): PRJNA335753

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Genome-wide transcriptional analysis of PTTH-stimulated Bombyx prothoracic gland

Project description:This is part of a cross-species approach to characterize transcriptional response of the insect prothoracic gland (PG) to the prothoracicotropic hormone (PTTH). Genome-wide response of the PG to PTTH was analyzed in vitro in Bombyx and in vivo in Drosophila, and the results were compared to identify common components in the PTTH signaling pathway. Bombyx PGs were incubated in vitro for 1 or 3 hours with or without 10 nM recombinant PTTH. 12-18 larvae were used to prepare each RNA sample, and the experiment was conducted twice. Total RNA samples from two independent preparations were combined to obtain a single RNA sample for each treatment, which was subjected to RNA-seq.

2016-07-29 | E-GEOD-84963 | biostudies-arrayexpress

Proteomic analysis of prothoracic glands of the silkworm Bombyx mori

Project description:The project aim was to identify the cell membrane receptors that prothoracic gland cells use to decode and transduce information from the extracellular environment during the final larval stage of the model insect, Bombyx mori.

2016-01-27 | PXD002771 | Pride

Proteomic analysis of Bombyx mori prothoracic glands from day 0 and day 6 of the 5th instar

Project description:The project aim was to identify the proteins expressed by the prothoracic gland cells during the final larval stage of the model insect, Bombyx mori.

2016-06-08 | PXD004265 | Pride

Bombyx mori

Project description:Genome-wide transcriptional analysis of PTTH-stimulated Bombyx prothoracic gland

| PRJNA335753 | ENA

Genome-winding binding profile of KDM5 in Drosophila larval prothoracic gland cells via Targeted DamID analysis

Project description:kdm5 is an essential gene in Drosophila that has critical developmental roles in the prothoracic gland cells of the larval ring gland. To profile KDM5 binding within these cells and this developmental stage, we performed Targeted DamID (TaDa). By profiling the nearest genes to significant TaDa peaks (FDR < 0.01), we identified 5815 candidate KDM5 target genes. Interestingly, 42% of these candidate KDM5 target genes appear to be conserved across multiple cellular contexts in Drosophila and span many cellular processes for future investigation.

2023-10-16 | GSE229076 | GEO

Bombyx mori Posterior and Median Silk Gland SAGE transcriptome

Project description:To identify functions that distinguish the posterior and median cells producing fibroin and sericin in the silk gland of Bombyx mori, serial analysis of gene expression (SAGE) profiles from both silk gland regions were analyzed and compared. The construction of a B. mori reference tag collection extracted from a set of 38000 Bombyx EST sequenced from the 3’ side, helped annotating the SAGE libraries. Most of the tags appeared at similar relative concentration in the two libraries except for those corresponding to silk proteins that were found region-specific and highly abundant. Strikingly, besides tags from silk protein mRNAs, 19 tags were found in the class of high abundance in the median cell library, which were absent in the posterior cell tag collection. Except tags from SP1 mRNA, no PSG specific tags were found in the same class of abundance. The analysis of MSG-specific different transcripts led to suggest that middle silk gland cell realizes more diversified functions as those already known, of synthesis and secretion of the silk sericins.

2010-12-22 | GSE23098 | GEO

The insect prothoracic gland as a model for steroid hormone biosynthesis and regulation

Project description:The insect prothoracic gland as a model for steroid hormone biosynthesis and regulation

| PRJNA319036 | ENA

Project description:Transcriptomes of silkworm Bombyx mori prothoracic gland (PG) at L5D1 and W0

| PRJNA916687 | ENA

Bombyx mori strain:p50T

Project description:Transcriptome analysis of prothoracic gland and brain in the silkworm Bombyx mori

| PRJDB2915 | ENA

Mitochondrial homeostasis of endocrine organ navigates nutrient restriction checkpoint

Project description:Steroid hormones are powerful endocrine regulators, but little is known about the integration of their production with environmental conditions. Here, we use Drosophila prothoracic gland (PG) to examine how insect steroid hormone production navigates a developmental checkpoint critical for juvenile-to-adult transition. Extensive transcriptome analysis of the PGs revealed that starvation during pre-nutrient restriction checkpoint (NRC) downregulated substantial mitochondria-related genes. We then discovered that pre-NRC starvation reduced prothoraciotropic hormone signaling, insulin signaling, and TORC1 activity in PG cells, which prevented mitochondrial fragmentation and import of Disembodied, a key steroidogenic enzyme. Ultimately, this starvation caused severe mitophagy and proteasome dysfunction, blocking ecdysteroidgenesis and metamorphosis. By contrast, post-NRC starvation did not impair mitochondrial homeostasis in PG cells, but reduced Sit expression and induced moderate autophagy to promote ecdysteroidgenesis, leading to precocious metamorphosis. This study constitutes a paradigm for exploring how steroid hormones themselves are controlled in response to environmental stress like starvation.

2024-12-02 | GSE267248 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data