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Perturbation of epigenetic processes by doxorubicin in the mouse testes: mRNA analysis

ABSTRACT: mRNA profiling of mouse testis comparing treatment with doxorubicin to vehicle only control (published in Toxicology Research, DOI: 10.1039/C6TX00078A)

ORGANISM(S): Mus musculus

PROVIDER: GSE85140 | GEO | 2016/08/04

SECONDARY ACCESSION(S): PRJNA336320

REPOSITORIES: GEO

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Perturbation of epigenetic processes by doxorubicin in the mouse testes: microRNA analysis

Project description:microRNA profiling of mouse testis comparing treatment with doxorubicin to vehicle only control (published in Toxicology Research, DOI: 10.1039/C6TX00078A)

2016-06-24 | GSE83661 | GEO

Perturbation of epigenetic processes by doxorubicin in the mouse testes: microRNA analysis

Project description:microRNA profiling of mouse testis comparing treatment with doxorubicin to vehicle only control (published in Toxicology Research, DOI: 10.1039/C6TX00078A) Two-condition experiment, doxorubicin vs control at 3 time points (1wk, 4wk and 7 wk following treatment). Biological replicates: 12 control, 12 doxorubicin (implementing a dye swap design of n=6 forward and n=6 reverse). One replicate per dual colour array (total of 36 arrays).

2016-06-24 | E-GEOD-83661 | biostudies-arrayexpress

Perturbation of epigenetic processes by doxorubicin in the mouse testes: mRNA analysis

Project description:mRNA profiling of mouse testis comparing treatment with doxorubicin to vehicle only control (published in Toxicology Research, DOI: 10.1039/C6TX00078A) Two-condition experiment, doxorubicin vs control at 3 time points (1wk, 4wk and 7 wk following treatment). Biological replicates: 6 control, 6 doxorubicin (implementing a dye swap design of n=3 forward and n=3 reverse). One replicate per dual colour array, which consists of two slides (total of 18 arrays and 36 slides/raw data files).

2016-08-04 | E-GEOD-85140 | biostudies-arrayexpress

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Perturbation of epigenetic processes by doxorubicin in the mouse testis.

Project description:Epigenetic processes play a major role in normal mammalian development, particularly during gametogenesis and early embryogenesis. Thus, perturbation of epigenetic processes in the testis by xenobiotics could have a major impact on testicular function and fertility, and potentially affect the development and health of subsequent generations. There has been substantial research into the epigenetic toxicity of environmental exposures over the last decade. However, few studies have focussed on pharmaceutical drugs, which due to the nature of their use are typically found at much higher concentrations within exposed individuals than environmental chemicals. Here, we investigated genome-wide changes in testicular mRNA transcription, microRNA expression and DNA methylation to assess the contribution of epigenetic mechanisms to the testicular toxicity induced by doxorubicin (DOX) as a representative, widely used and well-characterised anti-cancer drug. We demonstrated that DOX is able to induce transcriptional, microRNA and DNA methylation changes, which perturb pathways involved in stress/cell death and survival and testicular function and lead to germ cell loss and reproductive organ damage. This identified potential novel mechanisms of DOX-induced testicular toxicity for further focussed investigations. Such work is required to fully assess the role of epigenetics in toxicity, determine whether single and/or multigenerational epigenetic toxicity is a real public health concern, and begin to develop and incorporate relevant epigenetic endpoints into regulatory toxicology.

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Effects on gene expression of doxorubicin in human stem cells-derived cardiomyocytes

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