Transcriptomics

Dataset Information

0

Comparison of gene expression response to neutron and x-ray irradiation using mouse blood


ABSTRACT: Background: In the event of an improvised nuclear device detonation, the prompt radiation exposure would consist of γ rays plus a neutron component that would contribute to the total dose. As neutrons cause more complex and difficult to repair damage to cells that would result in more severe health burden to affected individuals, it is paramount to be able to estimate the contribution of neutrons to an estimated dose, to provide information for those making treatment decisions. Results: Mice exposed to either 0.25 or 1 Gy of neutron or 1 or 4 Gy x-ray radiation were sacrificed at 1 or 7 days after exposure. Whole genome microarray analysis identified 7,285 and 5,045 differentially expressed genes in the blood of mice exposed to neutron or x-ray radiation, respectively. Neutron exposure resulted in mostly downregulated genes, whereas x-rays showed both down- and up-regulated genes. A total of 34 differentially expressed genes were regulated in response to all ≥1 Gy exposures at both times. Of these, 25 genes were consistently downregulated at days 1 and 7, whereas 9 genes, including the transcription factor E2f2, showed bi-directional regulation; being downregulated at day 1, while upregulated at day 7. Gene ontology analysis revealed that genes involved in nucleic acid metabolism processes were persistently downregulated in neutron irradiated mice, whereas genes involved in lipid metabolism were upregulated in x-ray irradiated animals. Most biological processes significantly enriched at both timepoints were consistently represented by either under- or over-expressed genes. In contrast, cell cycle processes were significant among down-regulated genes at day 1, but among up-regulated genes at day 7 after exposure to either neutron or x-rays. Cell cycle genes downregulated at day 1 were mostly distinct from the cell cycle genes upregulated at day 7. However, five cell cycle genes, Fzr1, Ube2c, Ccna2, Nusap1, and Cdc25b, were both downregulated at day 1 and upregulated at day 7. Conclusions: We describe, for the first time, the gene expression profile of mouse blood cells following exposure to neutrons. We have found that neutron radiation results in both distinct and common gene expression patterns compared with x-ray radiation.

ORGANISM(S): Mus musculus

PROVIDER: GSE85323 | GEO | 2017/02/01

SECONDARY ACCESSION(S): PRJNA338172

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2018-06-15 | GSE113509 | GEO
2017-02-22 | GSE90909 | GEO
2020-09-02 | GSE113611 | GEO
2010-05-17 | E-GEOD-19526 | biostudies-arrayexpress
2014-09-26 | E-GEOD-55954 | biostudies-arrayexpress
2014-09-26 | E-GEOD-55953 | biostudies-arrayexpress
2018-02-21 | E-MTAB-5761 | biostudies-arrayexpress
2011-02-04 | E-GEOD-24876 | biostudies-arrayexpress
2010-11-16 | E-GEOD-25343 | biostudies-arrayexpress
2010-08-05 | E-GEOD-23393 | biostudies-arrayexpress