APOL1 renal-risk variants induce mitochondrial dysfunction (Illumina)
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ABSTRACT: To elucidate pathways whereby apolipoprotein L1 gene (APOL1) G1 and G2 variants facilitate kidney disease in African Americans, human embryonic kidney cells (HEK293) were used to establish doxycycline-inducible (Tet-on) cell lines stably expressing reference APOL1 G0 and its G1 and G2 renal-risk variants. Illumina human HT-12-v4 arrays and Affymetrix HTA 2.0 arrays were employed to generate global gene expression data with doxycycline induction. Significantly altered pathways identified through bioinformatics involved mitochondrial function; results were validated using immunoblotting, immunofluorescence and functional assays.
ORGANISM(S): Homo sapiens
PROVIDER: GSE85918 | GEO | 2016/08/23
SECONDARY ACCESSION(S): PRJNA339760
REPOSITORIES: GEO
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