Testis transcriptional profiling of males deriving from two high fertility mouse lines (FL1, FL2) which have been long-term selected for the characteristics large and heavy litters in comparison to randomly selected control line (Ctrl)

ABSTRACT: As generally known, reproduction is referred to as a couple’s challenge. Many genes generally coding for reproduction performance are shared by both sexes. For investigating the impact of ‘high fertility’ breeding on murine males testis transcriptomic profile was examined of two worldwidely unique outbred long-term mouse lines which have been selected for large and heavy litters - Fertility Lines 1 and 2 (FL1, FL2); also known as DUK and DUC. After >170 generations of generally female focused breeding both high fertile mouse lines dramatically increased the number of offspring as well as the total litter weight up to 180%, which is additionally accompanied by raised ovulation rate as could be shown by previous data. Concomitantly with the improved fertility phenotype, for the FL males several line-specific phenotypic characteristics could already be revealed like physiological parameters (e.g. higher bodyweight, accelerated puberty and life expectancy), behavioural parameters (e.g. explorative behaviour in an open field and decreased endurance fitness on a treadmill) or testicular cell type composition (e.g. altered haploid and diploid cell content shown by flow cytometric analysis). By this combination of phenotypic characterization and profound transcriptomic profiling we hope to ensure for future perspective that these genetically heterogeneous mouse models will provide new insights into molecular and cellular mechanisms of enhancing male fertility.

ORGANISM(S): Mus musculus

PROVIDER: GSE86063 | GEO | 2017/06/30

SECONDARY ACCESSION(S): PRJNA340159

REPOSITORIES: GEO