Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-Seq analyses of liver samples in mice fed ad libitum or subject to a 24 hr fast

ABSTRACT: To systemically identify long non-coding RNAs (lncRNAs) regulating energy metabolism, we performed transcriptome analyses to simultaneously profile mRNAs and lncRNAs in key metabolic organs in mice under pathophysiologically representative metabolic conditions by using a microarray platform (Mouse LncRNA Array V2.0, Arraystar). This microarray platform has been thoroughly evaluated for detecting mRNAs, but to further confirm its accuracy in detecting lncRNA expression at a genome-wide level, we performed RNA-seq for the same ad libitum and fasting liver samples used for the microarray. For those differentially expressed lncRNAs (fasting vs ad libitum) identified by microarray, 93.4% could be detected by RNA-seq and the Pearson correlation between microarray and RNA-seq of expression changes for these lncRNAs is 0.71.

ORGANISM(S): Mus musculus

PROVIDER: GSE86371 | GEO | 2016/09/22

SECONDARY ACCESSION(S): PRJNA341523

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Microarray of liver from Red Junglefowl (Gallus gallus) raised on intermittent fasting, chronic 70% restriction or ad libitum feeding

Project description:To better understand the hepatic metabolic response to intermittent fasting in chickens, Red Junglefowl chickens were raised on ad libitum (AL) feed until 14 days of age and then kept on AL feeding, switched to chronic feed restriction (CR) to around 70% or switched to an intermittent fasting (IF) regimen consisting of two fed days (150% of age-matched weight-specific AL intake offered daily) followed by a non-fed day. AL and CR were culled at 36 days of age, and IF birds either at 40 days of age (second consecutive feeding day) or 41 days of age (fasting day).

2022-04-14 | E-MTAB-7829 | biostudies-arrayexpress

Effects of ad libitum feeding on canine skeletal muscle gene expression

Project description:Skeletal muscle, as a large and insulin sensitive tissue, is an important contributor to metabolic homeostasis and energy expenditure. Obese dogs exhibit skeletal muscle insulin resistance, but the causes of these changes are unclear. Thus, we used canine microarrays to analyze gene expression profiles of skeletal muscle tissue collected from obese dogs, after 24 wk of ad libitum feeding. Skeletal muscle tissue samples were collected from 9 intact female beagles (4 yr-old; 4 control; 5 ad libitum) after 24 wk of ad libitum feeding.

2012-01-15 | E-GEOD-29279 | biostudies-arrayexpress

Development of gene expression signatures during fasting and refeeding (compensatory growth) for zebrafish liver

Project description:Here, we certified zebrafish to be a suitable model for the compensatory growth study. After a 3-weeks fasting, recovered for 3 days, 10 days and 15 days were chose as the initial, middle and late sampling sites for compensatory growth analysis, and liver samples of each time point were chose for the microarray analysis.At the same time, zebrafish fed ad libitum, as well as fasting for 3 weeks were also added to the microarray research.

2018-03-24 | GSE112272 | GEO

Global hepatic gene expression data from PPARa liver KO, PPARa liver WT, PPARaKO and WT male mice fed ad libitum, fasted for 24 hours and re-fed

Project description:If the function of the nuclear receptor PPARa is well-known during a prolongated fasting, its hepatic biological function during feeding and refeeding conditions still needs to be investigated. Moreover, in vivo data collected so far on PPARa function during fasting were obtained using the total Ppara KO transgenic mouse model. To identify genes whose expression is under the strict dependence of hepatic PPARa activity, we generated a new mouse strain of PPARa-specific deletion in hepatocyte (albumin-Cre+/- Pparaflox/flox or LKO) and we compared them to total Ppara KO (KO), wild-type (WT) and liver WT (albumin-Cre-/- Pparaflox/flox or LWT) mice under three nutritional challenges. We used microarrays to detail the global programme of gene expression in liver of Ppara LKO, LWT, Ppara KO and WT male mice fed ad libitum, fasted for 24 hours and refed. There are 52 liver samples, each from an individual mouse. The samples are from Ppara liver KO (LKO), Ppara KO (KO), wild-type (WT) and liver WT (LWT) male mice of 8 week-old from the same genetic background (C57Bl/6J) fed ad libitum, fasted for 24 hours, fasted for 24 hours and then refed 24 hours more with glucose added in water (200g/l). In fed condition (Fed), n= 3 mice for LKO, LWT genotypes, n= 5 for KO and n= 4 fot WT; in fasting condition (Fas), n=5 for LKO, LWT and WT genotypes and n= 3 for KO; in refeeding condition (Ref), n= 5 for LKO, KO and WT genotypes and n= 4 for LWT. All mice were sacrified at ZT14.

2016-02-20 | E-GEOD-73299 | biostudies-arrayexpress

Expression data from ad libitum or restricted fed 16-week old broiler breeder hens

Project description:Ovarian dysfunction is observed in broiler breeder hens fed ad libitum that is most apparent by a reduction in fertility success. Restricting caloric intake in these hens is currently the only effective treatment to improve reproductive efficiency. This analysis sought to determine the transcriptional changes in the ovarian cortex of prepubertal hens given full access to feed (FF) or were placed on a restricted feed (RF) diet. Microarrays were employed to detail the differences in global gene expression that underlie the ovarian dysfunction observed in ad libitum fed hens.

2021-09-15 | GSE184105 | GEO

Effects of preoperative fasting on renal ischemia-reperfusion injury in young-lean and aged overweight mice

Project description:To study the protective effects of preoperative fasting against renal ischemia-reperfusion injury, young-lean as well as aged overweight mice were subjected to three days of fasting or ad libitum food consumption, and gene expressions in kidneys of male mice were analyzed

2014-06-03 | GSE52982 | GEO

Expression data from Paneth cells isolated from mice on calorie restricted or ad libitum diet

Project description:Paneth cells recide in the intestinal crypt bottom and are part of the innate immunity and of the intestinal stem cell niche. We used microarrays to detail the global changes in gene expression following reduced calorie intake. Mice were kept on ad libitum or calorie restricted (60% of calories of ad libitum) diets for 4-7 weeks and paneth cells were isolated using flowcytometry

2012-04-11 | E-GEOD-37209 | biostudies-arrayexpress

Gene expression data of livers from C57BL/6J mice fed ad libitum or were fasted for 16 hour.

Project description:We report the RNA-Sequencing data for the livers of C57BL/6J mice fed ad libitum or were fasted for 16 hour.

2021-02-26 | GSE159038 | GEO

Effects of ad libitum feeding on canine subcutaneous adipose tissue gene expression

Project description:Adipose, once considered an inert storage depot, is now known to be an active endocrine tissue involved in total body homeostasis and metabolism, which exerts effects on multiple systems including food intake, immune function, and blood glucose regulation. During the development of obesity, adipose undergoes major expansion and remodeling, but the biological processes involved in this transition are not well understood. Thus, we used canine microarrays to analyze gene expression profiles of subcutaneous adipose tissue during the transition from a lean to obese phenotype. Subcutaneous adipose tissue samples were collected from 9 intact female beagles (4 yr-old; 4 control; 5 ad libitum) at baseline, 4, 8, 12 and 24 wk after beginning ad libitum feeding.

2010-09-14 | E-GEOD-24099 | biostudies-arrayexpress

Global hepatic gene expression data from PPARa liver KO, PPARa liver WT, PPARaKO and WT male mice fed ad libitum, fasted for 24 hours and re-fed

2016-02-20 | GSE73299 | GEO