Transcriptomics

Dataset Information

0

Systematic mapping of functional enhancer-promoter connections with CRISPR interference


ABSTRACT: Gene expression in mammals is regulated by noncoding elements that can impact physiology and disease, yet the functions and target genes of most noncoding elements remain unknown. We present a high-throughput approach that uses CRISPR interference (CRISPRi) to discover regulatory elements and identify their target genes. We assess >1 megabase (Mb) of sequence in the vicinity of 2 essential transcription factors, MYC and GATA1, and identify 9 distal enhancers that control gene expression and cellular proliferation. Quantitative features of chromatin state and chromosome conformation distinguish the 7 enhancers that regulate MYC from other elements that do not, suggesting a strategy for predicting enhancer-promoter connectivity. This CRISPRi-based approach can be applied to dissect transcriptional networks and interpret the contributions of noncoding genetic variation to human disease.

ORGANISM(S): Homo sapiens

PROVIDER: GSE87257 | GEO | 2016/11/11

SECONDARY ACCESSION(S): PRJNA343954

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2014-02-24 | E-GEOD-52830 | biostudies-arrayexpress
2024-01-08 | E-MTAB-13324 | biostudies-arrayexpress
2014-02-24 | GSE52830 | GEO
2023-11-14 | GSE225157 | GEO
2024-03-06 | GSE222297 | GEO
2024-03-06 | GSE222294 | GEO
2019-10-21 | GSE118843 | GEO
2019-11-01 | GSE124193 | GEO
2019-10-21 | GSE118911 | GEO
2019-10-21 | GSE124191 | GEO