Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MiRNA expression data from mice Ago2-amygdala

ABSTRACT: In this study we aimed to uncover which miRNAs are changed in the Ago2-amygdala complex following chronic social defeat in mice We used microarrays to determine which miRNAs are changed in the Ago2-amygdala complexof mice following chronic social defeat compared to control

ORGANISM(S): synthetic construct Mus musculus

PROVIDER: GSE87488 | GEO | 2016/09/30

SECONDARY ACCESSION(S): PRJNA344901

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression data from mice Ago2-amygdala

Project description:In this study we aimed to uncover which mRNAs are changed in the Ago2-amygdala complex following chronic social defeat in mice We used microarrays to determine which mRNAs are changed in the Ago2-amygdala complexof mice following chronic social defeat compared to control

2016-09-30 | GSE87489 | GEO

Gene expression analysis of mouse BNST and blood cells after chronic social defeat stress (CSDS)

Project description:Chronic psychosocial stress is a risk factor for psychiatric disorders, and genetic factors interact in conferring susceptibility. The chronic social defeat stress (CSDS) mouse model allows identifying factors underlying resilience and susceptibility to chronic psychosocial stress. Following 10 days of CSDS, we collected the brain and blood tissues. We performed (1) AGO2 RNA immunoprecipitation-sequencing (AGO2 RIP-seq) of active miRNAs and their mRNA targets in the BNST. Additionally, we carried out RNA-seq and miRNA-seq from blood cells.

2019-08-15 | GSE122840 | GEO

Small RNA Sequencing of mouse amygdala after knockdown of miR-15a

Project description:MicroRNAs are important regulators of gene expression and associated with stress-related psychiatric disorders. We report that exposing mice to chronic stress led to a specific increase in microRNA-15a levels in the amygdala-Ago2 complex, and a concomitant reduction in the levels of its predicted target, FKBP51, which is implicated in stress-related psychiatric disorders. Reciprocally, mice expressing reduced levels of amygdalar microRNA-15a following exposure to chronic stress exhibited increased anxiety-like behaviors. Here, we performed small RNA Sequencing of mouse basolateral amygdala after miR15a knockdown using injection of a miR-15a sponge virus or control sponge virus.

2016-10-01 | GSE87533 | GEO

Proteomic and transcriptional profiling of rat amygdala following social play

Project description:Social play was performed on one cohort of adolescent rats. Other cohort was remaining naïve to behavioral testing. The rats were scarified following behavioral testing on postnatal day 38. Amygdala was isolated form the brain, homogenized the tissue, proteins were quantified, trypsin digested, and analyzed by LC ESI MS/MS.

2019-11-06 | PXD012677 | Pride

mRNAs associated with Ago2: Ago2, Ago2+miR-1, Ago2+miR-124, mock transfected

Project description:As a core RISC component, Ago2 associates with miRNAs and target mRNAs. To identify these mRNAs, we ran lysate from HEK293T cells over a FLAG resin from 2 conditions: +FLAG-Ago2, +mock transfection. To identify mRNAs associated with specific miRNAs, we ran lysate from HEK293T cells over a FLAG resin from 2 conditions: +FLAG-Ago2 & miR-1, and +FLAG-Ago2 & miR-124. Set of arrays that are part of repeated experiments Compound Based Treatment: mock transfected Keywords: Biological Replicate

2008-04-08 | GSE11079 | GEO

Rat, male, brain, medial amygdala, LC-MSMS

Project description:Social isolation poses a severe mental and physiological burden on humans. Most animal models that investigate this effect are based on prolonged isolation, which does not mimic the milder conditions experienced by people in the real world. Here we show that in the medial amygdala, a brain structure that is crucial for social memory, acute social isolation causes social memory loss and significant changes in specific mRNAs and proteins.

2021-11-03 | PXD026871 | Pride

miRNA arrays: Ago2 transfected vs. Mock, Ago2 IP vs. total RNA

Project description:miRNAs were enriched from HEK293T cells using the ambion FLASHPAGE fractionater after mock transfection, Ago2 transfection, and FLAG-Ago2 IP. miRNAs were labeled and hybridized: Ago2 transfected vs. mock transfection and Ago2 transfected vs. Ago2 IPs. Set of arrays that are part of repeated experiments Keywords: Biological Replicate

2008-04-08 | GSE11081 | GEO

mRNAs associated with Ago2: Ago2, Ago2+miR-1, Ago2+miR-124, mock transfected

2008-04-08 | E-GEOD-11079 | biostudies-arrayexpress

microRNA binding by wildtype Ago2 and mutant Ago2 in human cells

Project description:Ago2 binds mature microRNAs (miRNAs) to regulate gene expression. A mutant Ago2 is constructed to render it unable to bind miRNAs and inhibit mRNA translation.

2013-03-01 | GSE37941 | GEO

Proteomic and transcriptional profiling of rat amygdala following social play

Project description:Proteomic and transcriptional profiling of rat amygdala following social play

| PRJNA518911 | ENA