Transcriptomics

Dataset Information

0

Ribosome profiling of dhh1∆ yeast


ABSTRACT: The impact of RNA structures in coding sequences (CDS) within mRNAs is poorly understood. Here 
we identify a novel and highly conserved mechanism of translational control involving RNA structures within coding sequences and the DEAD-box helicase Dhh1. Using yeast genetics and 
genome-wide ribosome profiling analyses we show that this mechanism, initially derived from studies 
of the Brome Mosaic virus RNA genome, extends to yeast and human mRNAs highly enriched in 
membrane and secreted proteins. All Dhh1-dependent mRNAs, viral and cellular, share key common 
features. First, they contain long and highly structured CDSs, including a region located around 42 to 
120 nucleotides after the translation initiation site, second, they are directly bound by Dhh1 with a 
specific binding distribution and third, complementary experimental approaches suggest that they are 
activated by Dhh1 at the translation initiation step. Our results show that ribosome translocation is not the only unwinding force of CDS and uncover a conserved layer of translational control that involve 
RNA helicases and RNA folding within CDS providing novel opportunities for regulation of 
membrane and secretome proteins.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE87892 | GEO | 2016/11/04

SECONDARY ACCESSION(S): PRJNA348115

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-11-04 | GSE87888 | GEO
2023-10-24 | PXD042990 | Pride
2023-10-24 | PXD042828 | Pride
2024-10-08 | GSE270789 | GEO
2024-10-08 | GSE270790 | GEO
2016-05-11 | GSE81269 | GEO
2014-09-08 | GSE60983 | GEO
2014-09-08 | E-GEOD-60983 | biostudies-arrayexpress
2019-02-27 | GSE114892 | GEO
2023-09-27 | GSE224774 | GEO