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Improved post thaw function and genetic changes for mesenchymal stromal cells cryopreserved using multicomponent osmolyte solutions

ABSTRACT: We report genome-wide expression changes that occur in H9-iMSCs frozen with different freezing methods that include DMSO and non-DMSO experimental solutions such as SGC (sucrose-glycerol-creatinine, SMC (sucrose-mannitol-creatinine), and SGI (sucrose-mannitol-isoleucine). mRNA-Seq analysis shows that DMSO samples cluster with fresh samples in the same clade, while all samples using the experimental solutions cluster together. In addition, we also see that cells frozen using experimental solutions have upregulation of a number of key molecular function pathways including extracellular matrix structural genes, receptor binding, and growth factor expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE88946 | GEO | 2016/12/30

SECONDARY ACCESSION(S): PRJNA349169

REPOSITORIES: GEO

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