Transcriptomics

Dataset Information

0

Transcriptomic screen to identify genes regulated by Store-operated calcium entry in Drosophila pupal nervous system


ABSTRACT: Transcriptional regulation by Store-operated Calcium Entry (SOCE) is well studied in non-excitable cells. However, the role of SOCE has been poorly documented in neuronal cells with more complicated calcium dynamics. Previous reports demonstrated a requirement of neuronal SOCE for Drosophila flight. We identified the early pupal stage to be critical and used RNA-sequencing to identify SOCE mediated gene expression changes in the developing Drosophila pupal nervous system. We down-regulated dStim, the endoplasmic reticular calcium sensor and a principal component of SOCE in the nervous system for a 24h period during pupal development, and compared wild type and knockdown transcriptional profiles, immediately after knockdown as well as after a 36h recovery period. We found that dStim knockdown altered the expression of a number of genes. We also characterized one of the down-regulated genes, Ral for its role in flight. Thus, we identify neuronal SOCE as a mechanism that regulates expression of a number of genes during the development of the pupal nervous system. These genes can be further studied in the context of pupal nervous system development.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE89168 | GEO | 2017/03/21

SECONDARY ACCESSION(S): PRJNA350516

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-09-01 | GSE157200 | GEO
2018-06-27 | GSE109111 | GEO
2023-08-07 | GSE226570 | GEO
2023-11-01 | GSE246431 | GEO
2016-03-10 | E-GEOD-79039 | biostudies-arrayexpress
2016-03-10 | GSE79039 | GEO
| PRJNA350516 | ENA
2020-01-11 | GSE143430 | GEO
2007-08-03 | E-GEOD-1309 | biostudies-arrayexpress
2023-12-09 | E-MTAB-13548 | biostudies-arrayexpress