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Drosophila melanogaster

ABSTRACT: Transcriptomic screen to identify genes regulated by Store-operated calcium entry in Drosophila pupal nervous system

PROVIDER: PRJNA350516 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4449930.fastq.gz	Fastqsanger.gz
	SRR4449931.fastq.gz	Fastqsanger.gz
	SRR4449932.fastq.gz	Fastqsanger.gz
	SRR4449933.fastq.gz	Fastqsanger.gz
	SRR4449934.fastq.gz	Fastqsanger.gz

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Similar Datasets

Transcriptomic screen to identify genes regulated by Store-operated calcium entry in Drosophila pupal nervous system

Project description:Transcriptional regulation by Store-operated Calcium Entry (SOCE) is well studied in non-excitable cells. However, the role of SOCE has been poorly documented in neuronal cells with more complicated calcium dynamics. Previous reports demonstrated a requirement of neuronal SOCE for Drosophila flight. We identified the early pupal stage to be critical and used RNA-sequencing to identify SOCE mediated gene expression changes in the developing Drosophila pupal nervous system. We down-regulated dStim, the endoplasmic reticular calcium sensor and a principal component of SOCE in the nervous system for a 24h period during pupal development, and compared wild type and knockdown transcriptional profiles, immediately after knockdown as well as after a 36h recovery period. We found that dStim knockdown altered the expression of a number of genes. We also characterized one of the down-regulated genes, Ral for its role in flight. Thus, we identify neuronal SOCE as a mechanism that regulates expression of a number of genes during the development of the pupal nervous system. These genes can be further studied in the context of pupal nervous system development.

2017-03-21 | GSE89168 | GEO

Chronic dysfunction of Store-operated calcium entry by pulsed RNAi induction in fat tissue impairs organismal energy homeostasis in Drosophila

Project description:The purpose is to have a overview on how chronic dysfunction of store-operated calcium entry change transcription profiles of adult fly fat tissue. Methods:To achieve chronic dysfunction of store-operated calcium entry, we carried out the Temperature-caused RNAi Pulse Induction of Stim (29 celcius degree incubation, Stim-TRiPI, or Stim-TRIP in data file) in fat storage tissue of 6 days old adult male flies. At day 10 and 11 after Stim-TRiPI (Stim-TRIP), we isolated the fat tissues and carried out RNAseq analysis based gene-level read counts.

2019-05-07 | GSE112689 | GEO

Selective modulation of gene expression in activated normal human peripheral blood mononuclear cells by store-operated calcium entry blocker BTP2

Project description:Calcium is a critical signaling molecule in many cell types including immune cells. The calcium-release activated calcium channels (CRAC) responsible for store-operated calcium entry (SOCE) in immune cells are gated by STIM family members functioning as sensors of Ca2+ store content in the endoplasmic reticulum. We investigated the effect of SOCE blocker BTP2 on human peripheral blood mononuclear cells (PBMC) stimulated with the mitogen phytohemagglutinin (PHA).

2023-08-07 | GSE226570 | GEO

Differential regulation of membrane potential during store-operated calcium entry in neutrophil subsets

Project description:Calcium signals are initiated in immune cells by the process of store-operated calcium entry (SOCE), where receptor activation triggers transient calcium release from the endoplasmic reticulum, followed by opening of plasma membrane calcium-release activated calcium (CRAC) channels. ORAI1, ORAI2 and ORAI3 are known to comprise the CRAC channel, however the contributions of individual isoforms to neutrophil function is not well understood. Here we show that loss of ORAI1 partially decreases calcium influx while loss of both ORAI1 and ORAI2 completely abolishes store-operated calcium entry. In other immune cell types, loss of ORAI2 enhances SOCE. In contrast, we find that ORAI2-deficient neutrophils display decreased calcium influx, which is correlated with measurable differences in regulation of neutrophil membrane potential via KCa3.1. Decreased SOCE in ORAI1-, ORAI2- and ORAI1/2-deficient neutrophils impairs multiple neutrophil functions including phagocytosis, degranulation, leukotriene and ROS production, rendering ORAI1/2-deficient mice highly susceptible to staphylococcal infection. This study demonstrates that ORAI1 and ORAI2 are the primary components of the neutrophil CRAC channel and identifies novel subpopulations of neutrophils where cell membrane potential functions as a rheostat to modulate the SOCE response. These findings have implications for new mechanisms that modulate neutrophil function during infection, acute and chronic inflammatory conditions, and cancer.

2020-09-01 | GSE157200 | GEO

Differential regulation of membrane potential during store-operated calcium entry in neutrophil subsets

Project description:Differential regulation of membrane potential during store-operated calcium entry in neutrophil subsets

| PRJNA660437 | ENA

Orai-mediated calcium entry regulates the excitability of central dopaminergic neurons

Project description:Transcriptomic screen from Drosophila pupal CNS to identify genes regulated by loss of Orai-mediated Ca2+ entry in a subset of dopaminergic neurons by means of expression of the OraiE180A dominan negative allele

2024-01-25 | GSE230134 | GEO

Sustained store-operated calcium entry utilizing activated chromatin state leads to instability in iTregs

Project description:Sustained store-operated calcium entry utilizing activated chromatin state leads to instability in iTregs

| PRJNA1032926 | ENA

Homeostatic SOCE prevents complex cell stress responses

Project description:We report the application of bulk RNA-seq for detection of changes in the mRNA-transcriptome of cultured primary, cortical astrocytes when homeostatic calcium fluxes are modulated. We investigated three conditions: (1) control (2) glial cells treated with thapsigargin, a blocker of the SERCA. This causes the depletion of ER calcium and induces store-operated calcium entry (SOCE). (3) Calcium-free extracellular solution. This causes an acute interruption of homeostatic calcium influx and thereby also causes ER calcium depletion. The aim was to detect immediate changes in the cellular transcriptome. Therefore, we treated the cells for just 60 min with the different conditions. The data show that about 8% (calcium-free condition) or 16% (SERCA blockade) of the whole mRNA transcriptome is changed within one hour, when homeostatic calcium fluxes are affected. Regulated are biological processes associated with cell health, differentiation, metabolism, ER stress responses, gene expression, and many more processes.

2024-07-09 | GSE242678 | GEO

ORAI1 is required for T cell mediated allergic airway inflammation but not antiviral immunity in the lung

Project description:This study investigates the role of store-operated calcium entry (SOCE) in T cell-mediated immune responses to pulmonary influenza A virus (IAV) infection and allergic airway inflammation after immunization by house dust mite (HDM) allergens. We conducted a comparative gene expression analysis of antigen-specific CD4+ T cells from wildtype (WT) and Orai1fl/fl Cd4Cre mice that were adoptively transferred to TCRalpha knockout mice followed by sensitization / challenge with HDM or infection with influenza A virus (IAV) strain x31. Donor CD4+ T cells were isolated from the lungs of host mice at days 9 (IAV) and 14 (HDM), RNA was isolated and processed for bulk RNA sequencing.

2022-05-01 | GSE184228 | GEO

Mus musculus

Project description:Store operated Ca2+ entry (SOCE) controls ameloblast cell function and enamel development

| PRJNA361476 | ENA