Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transciptome profiling of RNaseIII null cells reconstituted with Drosha

ABSTRACT: To assess the impact of Drosha reconstitution on the overall transcriptome of RNaseIII null cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE89790 | GEO | 2017/06/28

SECONDARY ACCESSION(S): PRJNA353306

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transciptome profiling of NoDice and RNaseIII null cells prior to and after polyIC treatment

Project description:To assess the impact of endogenous RNaseIII nucleases on the overall transcriptome and the reseponse to dsRNA. We utilized CRISPR techology to engineer an RNaseIII null cell line using the NoDice 293T parental strain genearated the Cullen lab as the parental cell line.

2017-06-28 | GSE86610 | GEO

Transciptome profiling of NoDice and RNaseIII null cells prior to and after virus infection

Project description:To assess the impact of endogenous RNaseIII nucleases on the overall transcriptome and the response to virus infection. We utilized CRISPR techology to engineer an RNaseIII null cell line using the NoDice 293T parental strain generated in the Cullen lab as the parental cell line.

2017-06-28 | GSE85159 | GEO

Pri-miRNA identification by generating a Drosha null/conditional-null mouse model.

Project description:We report the ability of the Drosha null/conditional-null mouse model to enable the identification of pri-miRNA transcripts. The conditional-null allele of Drosha phenocopies the null allele both in mESC and in mice, upon conversion to the null state with Cre.

2015-02-09 | GSE55735 | GEO

Pri-miRNA identification by generating a Drosha null/conditional-null mouse model.

2015-02-09 | E-GEOD-55735 | biostudies-arrayexpress

SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA

Project description:Neural stem cell regulation is essential for the formation of the central nervous system and homeostatic neurogenesis in the adult mammalian brain. The RNAseIII Drosha, a key component of the miRNA microprocessor, plays a central role in regulating NSC maintenance partially through a miRNA-independent mechanism. Drosha controls mRNA expression levels by targeting and cleaving evolutionary conserved stem-loop hairpins located in the mRNAs of stem cell-related transcription factors. However, it is unknown how the Drosha-mediated endonucleolytic cleavage of mRNA is regulated. Here, we identify novel Drosha and NFIB interactors in hippocampal NSCs by in vitro pull-down assays followed by Mass Spectrometry. We unravel the RNA binding proteins implicated in Drosha-mediated regulation of neurogenesis and we find Scaffold Attachment Factor B1 to play a novel and essential role in NFIB mRNA regulation during neural stem cell differentiation.

2024-06-03 | PXD017677 | Pride

Small RNA sequencing of mock treated or Sindbis virus-infected wild type and RNaseIII knockout cells

Project description:To assess the impact of endogenous RNaseIII nucleases on the small RNA profile in the presence and absence of virus infection.

2017-06-28 | GSE98135 | GEO

Homo sapiens

Project description:Transciptome profiling of RNaseIII null cells reconstituted with Drosha

| PRJNA353306 | ENA

Transcriptome profiling of primary murine fibroblast response to IFNB with or without prior Drosha depletion

Project description:To assess the impact of Drosha on the response to IFNB

2017-06-28 | GSE96889 | GEO

Impact of two RNaseIII-like protein on the small RNA repertoire

Project description:RNaseIII enzymes catalyze the cleavage of double stranded RNA (dsRNA) and have diverse functions in RNA maturation. Arabidopsis RNASE THREE LIKE2 (RTL2) carries one RNaseIII and two dsRNA-binding (DRB) domains, and is the unique Arabidopsis RNAse III enzyme resembling the budding yeast siRNA-producing Dcr1 enzyme. Here we show that RTL2 modulates the production of siRNAs, and that this activity depends on both RNaseIII and DRB domains. However, RTL2 mode of action differs from that of Dcr1. Indeed, whereas Dcr1 directly cleaves dsRNAs into 23-nt siRNAs, RTL2 likely cleaves dsRNAs into molecules longer than siRNAs, which are subsequently processed by the DCL enzymes. Depending on the dsRNA considered, RTL2-mediated maturation either improves (RTL2- dependent loci) or reduces the efficiency of DCL processing (RTL2-sensitive loci). Because the vast majority of plant small RNAs are 24-nt siRNAs that guide DNA methylation at transposons and intergenic regions, RTL2 depletion mostly modifies DNA methylation in these regions. Nevertheless, 13% of the siRNA-producing loci affected by RTL2 depletion correspond to protein coding genes. We show that changes in 24-nt siRNA levels also affect DNA methylation levels at such loci, and inversely correlate with mRNA steady-state levels, thus implicating RTL2 in the control of protein-coding gene expression.

2015-10-01 | GSE71782 | GEO

DROSHA complementation experiments using small RNA sequencing

Project description:DROSHA complementation experiments were performed to check whether dsRBD of DROSHA recognizes the lower stem of pri-miRNAs and affects miRNA biogenesis.

2018-12-13 | GSE117603 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data