Transcriptomics

Dataset Information

0

Mechanisms of Immune Suppression Utilized by Canine Adipose and Bone Marrow Stem Cells


ABSTRACT: Comparison of gene expression profiles of canine adipose and bone marrow derived mesenchymal stem cells by microarray Mesenchymal stem cells (MSC) from rodents and humans have been shown to suppress T cells by distinct primary pathways, with NO-dependent pathways dominating in rodents and IDO-dependent pathways dominating in humans. However, the immune suppressive pathways utilized by canine MSC have not been as thoroughly studied, nor have BM-MSC and Ad-MSC been directly compared for their immune modulatory potency or pathway utilization. Therefore, canine BM-MSC and Ad-MSC were generated in vitro and their potency in suppressing T cell proliferation and cytokine production was compared, as well as differential gene expression. Mechanisms of T cells suppression were also investigated for both MSC types. We found that BM-MSC and Ad-MSC were roughly equivalent in terms of their ability to suppress T cell activation. However, the two MSC types used both shared and distinct biochemical pathways to suppress T cell activation. Adipose-derived MSC utilized, TGF-β signaling pathways and adenosine signaling to suppress T cell activation, whereas BM-MSC used cyclooxygenase TGF-β, and adenosine signaling pathways to suppress T cell activation. These results indicate that canine MSC are distinct from human and rodent MSC terms of their immune suppressive pathways, relying primarily on cyclooxygenase and TGF-β pathways for T cell suppression, rather than on NO or IDO-mediated pathways.

ORGANISM(S): Canis lupus familiaris

PROVIDER: GSE90449 | GEO | 2016/11/23

SECONDARY ACCESSION(S): PRJNA354633

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-04-05 | GSE176055 | GEO
2019-02-12 | PXD011643 | Pride
2009-09-15 | E-GEOD-17782 | biostudies-arrayexpress
2009-08-25 | GSE17782 | GEO
2022-10-03 | GSE199826 | GEO
2024-06-12 | GSE217506 | GEO
2012-12-30 | E-GEOD-30807 | biostudies-arrayexpress
2017-01-26 | GSE94081 | GEO
2023-06-19 | GSE200161 | GEO
2020-01-29 | PXD016322 | Pride