Locus-specific histone deacetylation using a synthetic CRISPR-Cas9-based HDAC
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ABSTRACT: Efforts to manipulate locus-specific histone acetylation to assess their causal role in gene expression and cellular and behavioural phenotypes have been impeded by a lack of experimental tools. The Cas9 nuclease has been adapted to target epigenomic modifications, but a detailed description of the parameters of such synthetic epigenome remodellers is still lacking. Here we describe a Cas9-based histone deacetylase (HDAC) and the design principles required to achieve locus-specific histone deacetylation. We assess its range of activity and specificity, and analyse target gene expression in two different cell types to investigate cellular context-dependent effects. Our findings demonstrate that the chromatin environment is an important element to consider when utilizing this synthetic HDAC.
ORGANISM(S): Mus musculus
PROVIDER: GSE91043 | GEO | 2017/07/09
SECONDARY ACCESSION(S): PRJNA356734
REPOSITORIES: GEO
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