Genomics

Dataset Information

0

Locus-specific histone deacetylation using a synthetic CRISPR-Cas9-based HDAC


ABSTRACT: Efforts to manipulate locus-specific histone acetylation to assess their causal role in gene expression and cellular and behavioural phenotypes have been impeded by a lack of experimental tools. The Cas9 nuclease has been adapted to target epigenomic modifications, but a detailed description of the parameters of such synthetic epigenome remodellers is still lacking. Here we describe a Cas9-based histone deacetylase (HDAC) and the design principles required to achieve locus-specific histone deacetylation. We assess its range of activity and specificity, and analyse target gene expression in two different cell types to investigate cellular context-dependent effects. Our findings demonstrate that the chromatin environment is an important element to consider when utilizing this synthetic HDAC.

ORGANISM(S): Mus musculus

PROVIDER: GSE91043 | GEO | 2017/07/09

SECONDARY ACCESSION(S): PRJNA356734

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

| PRJNA356734 | ENA
| PRJNA159705 | ENA
2024-08-05 | GSE269243 | GEO
2014-02-23 | E-GEOD-37378 | biostudies-arrayexpress
2014-02-23 | E-GEOD-37377 | biostudies-arrayexpress
2014-02-23 | E-GEOD-54909 | biostudies-arrayexpress
2014-02-23 | E-GEOD-37376 | biostudies-arrayexpress
2014-02-23 | E-GEOD-54912 | biostudies-arrayexpress
2014-02-23 | E-GEOD-54911 | biostudies-arrayexpress
2014-02-23 | E-GEOD-54910 | biostudies-arrayexpress