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The Histone Deacetylase Rpd3p Coordinates Transient Changes in Genomic Expression in Response to Acute Stress

ABSTRACT: To characterize the role of chromatin remodeling proteins in mediating stress-activated expression changes, whole-genome expression was followed in wild-type and mutant cells (including strains lacking the histone deacetylase Rpd3p, associated Rpd3p proteins, and the transcription factors Msn2p and Msn4p) responding to multiple stresses including heat shock, hydrogen peroxide treatment, and salt stress, as well as stress relief. Keywords: Timecourse and single timepoint expression studies of stress response in yeast

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE9108 | GEO | 2009/05/21

SECONDARY ACCESSION(S): PRJNA102639

REPOSITORIES: GEO

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The Histone Deacetylase Rpd3p Coordinates Transient Changes in Genomic Expression in Response to Acute Stress

Project description:To characterize the role of chromatin remodeling proteins in mediating stress-activated expression changes, whole-genome expression was followed in wild-type and mutant cells (including strains lacking the histone deacetylase Rpd3p, associated Rpd3p proteins, and the transcription factors Msn2p and Msn4p) responding to multiple stresses including heat shock, hydrogen peroxide treatment, and salt stress, as well as stress relief. Keywords: Timecourse and single timepoint expression studies of stress response in yeast Gene expression was done in duplicate for timecourses of stress response and in at least triplicate for single timepoint measurements. A total of 109 microarrays were done.

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Project description:Microarray time courses followed the response of 5 yeast strains to heat shock. Expression variation due to genetic, environmental, and genotype-by-environment interactions were identified. Keywords: Timecourse and single timepoint expression studies of stress response in yeast The genomic expression response to heat shock was measured in 5 different yeast strains over the course of 2 hours. Basal expression at 25C was also compared in 4 non-lab strains to the S288c refernce. All experiments were done in duplicate, for a total of 68 Samples.

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