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The Histone Deacetylase Rpd3p Coordinates Transient Changes in Genomic Expression in Response to Acute Stress

ABSTRACT: To characterize the role of chromatin remodeling proteins in mediating stress-activated expression changes, whole-genome expression was followed in wild-type and mutant cells (including strains lacking the histone deacetylase Rpd3p, associated Rpd3p proteins, and the transcription factors Msn2p and Msn4p) responding to multiple stresses including heat shock, hydrogen peroxide treatment, and salt stress, as well as stress relief. Keywords: Timecourse and single timepoint expression studies of stress response in yeast Gene expression was done in duplicate for timecourses of stress response and in at least triplicate for single timepoint measurements. A total of 109 microarrays were done.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Audrey Gasch

PROVIDER: E-GEOD-9108 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The histone deacetylase Rpd3p is required for transient changes in genomic expression in response to stress.

Alejandro-Osorio Adriana L AL Huebert Dana J DJ Porcaro Dominic T DT Sonntag Megan E ME Nillasithanukroh Songdet S Will Jessica L JL Gasch Audrey P AP

Genome biology 20090526 5

<h4>Background</h4>Yeast responding to stress activate a large gene expression program called the Environmental Stress Response that consists of approximately 600 repressed genes and approximately 300 induced genes. Numerous factors are implicated in regulating subsets of Environmental Stress Response genes; however, a complete picture of Environmental Stress Response regulation remains unclear. We investigated the role of the histone deacetylase Rpd3p, previously linked to the upstream regions ...[more]

PMID: 19470158

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