Transcriptomics

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Methanol treatment with 5 % for 2 h


ABSTRACT: The number and type of synthetic chemicals that are being produced worldwide continues to increase significantly. While these industrial chemicals provide numerous benefits, there is no doubt that some have potential to damage the environment and health. Toxicity must be evaluated and use must be carefully controlled and monitored in order to minimize potential damage. DNA microarray technology has become an important new technique in toxicology. We are using the yeast Saccharomyces cerevisiae as a model organism for toxicological study because it is a simple, fast-growing eukaryote that has been thoroughly characterized. To obtain varied toxicity-induced gene expression alteration profiles, we examined many kinds of chemicals and compared the gene expression profiles as chemical toxicity each other. Consequently, cells need to be exposed with same experimental cellular condition, semi lethal (IC50), respectively. In the case of methanol (CAS; 67-56-1), the exposure dose was decided as 5 % by growth curve with continuously diluted exposure. Methanol is one of the alcohols, and suspected to be developmental, gastrointestinal or liver, kidney, neuro-, respiratory toxicant. Keywords: stress response

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE9229 | GEO | 2007/11/01

SECONDARY ACCESSION(S): PRJNA102829

REPOSITORIES: GEO

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