2-Aminobenzimidazole treatment with 5 mM for 2 h

ABSTRACT: The number and type of synthetic chemicals that are being produced worldwide continues to increase significantly. While these industrial chemicals provide numerous benefits, there is no doubt that some have potential to damage the environment and health. Toxicity must be evaluated and use must be carefully controlled and monitored in order to minimize potential damage. DNA microarray technology has become an important new technique in toxicology. We are using the yeast Saccharomyces cerevisiae as a model organism for toxicological study because it is a simple, fast-growing eukaryote that has been thoroughly characterized. In order to evaluate toxicity by newly synthesized or mixture chemicals, toxicity-induced gene expression alteration profiles by known chemicals should be collected. In our study, cells need to be exposed with same experimental cellular condition, semi lethal (IC50), respectively. In the case of 2-aminobenzimidazole (CAS; 934-32-7), the exposure dose was decided as 5 mM by growth curve with continuously diluted exposure. 2-Aminobenzimidazole is one of the hydrolyzed substrate from benomyl. Benomyl is a fungicide that has a selectively toxic to micro-organisms and to invertebrates, especially earthworms. Benomyl binds to microtubules, interfering with cell functions such as meiosis and intracellular transportation. The selective toxicity of benomyl as a fungicide is possibly due to its heightened effect on fungal rather than mammalian microtubules. Keywords: stress response

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE9324 | GEO | 2007/11/01

SECONDARY ACCESSION(S): PRJNA102993

REPOSITORIES: GEO