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Gene expression profiles of RALD iPSCs cultured with or without bFGF

ABSTRACT: We investigated roles of KRAS on stemness maintenance in the context of induced pluripotent stem cells (iPSCs) using isogenic KRAS mutant (G13C/WT) and wild-type (WT/WT) iPSCs from the same RAS-associated autoimmune lymphoproliferative syndrome-like disease (RALD) patients. Microarray analysis was conducted to compare gene expression profiles of WT/WT and G13C/WT iPS cells cultured with or without bFGF. Expression of stemness markers including POU5F1 and NANOG, which is indicative of an undifferentiated state, was highly maintained in G13C/WT iPSCs, but not in WT/WT iPSCs, under bFGF depletion, indicating enhanced self-renewal of iPSCs by the oncogenic KRAS.

ORGANISM(S): Homo sapiens

PROVIDER: GSE94141 | GEO | 2018/03/31

REPOSITORIES: GEO

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Gene expression profiles of RALD iPSCs cultured with or without bFGF

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