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Expression data from fractionated SH-SY5Y cells

ABSTRACT: miRNA activity is typically associated with the cytoplasm, but a growing body of evidence suggests non-canonical roles for miRNA in the nucleus. We study miRNA dynamics in neurons and wanted to investigate this possibility in a human neuronal model. We used microarrays to investigate the miRNA and mRNA composition of the nuclear and cytoplasmic compartments of undifferentiated SH-SY5Y cells to identify miRNA-target relationships and networks.

ORGANISM(S): Homo sapiens

PROVIDER: GSE94484 | GEO | 2017/12/01

SECONDARY ACCESSION(S): PRJNA369855

REPOSITORIES: GEO

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miRNA expression data from fractionated SH-SY5Y cells

Project description:miRNA activity is typically associated with the cytoplasm, but a growing body of evidence suggests non-canonical roles for miRNA in the nucleus. We study miRNA dynamics in neurons and wanted to investigate this possibility in a human neuronal model. We used microarrays to investigate the miRNA composition of the nuclear and cytoplasmic compartments of undifferentiated SH-SY5Y cells.

2017-12-01 | GSE94483 | GEO

miRNA & mRNA expression data from fractionated SH-SY5Y cells

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miRNA profiling in HEV71-infected SH-SY5Y cells

Project description:MicroRNA (miRNA) has been highlighted in pathogen-host interactions, however, little is known about roles of miRNAs in neurological pathogenesis of human enterovirus 71 (HEV71) infections. In this study, the comprehensive miRNA expression profiling in HEV71-infected human neuroblastoma SH-SY5Y cells were performed to identify cellular miRNAs response to HEV71. A total of 69 miRNAs were differentially expressed in HEV71-infected SH-SY5Y cells compared to non-infected cells. These findings provide new information on the miRNA and mRNA profiles in HEV71 infection, which may serve as a basis for further investigation into the biological functions of miRNAs in the neurological pathogenesis of HEV71 infections. Human neuroblastoma SH-SY5Y cells were infected with HEV71. After infection, the cells were harvested and extracted total RNA for miRNA profiling by hybridization on Affymetrix microarrays. A total of 69 miRNAs were differentially expressed inHEV71-infected SH-SY5Y cells compared to non-infected cells.

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Gene expression during neuronal differentiation in two subtypes of SH-SY5Y

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ER stress impairs the insulin signaling pathway through mitochondrial damage in SH-SY5Y human neuroblastoma cells (part 2)

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ER stress impairs the insulin signaling pathway through mitochondrial damage in SH-SY5Y human neuroblastoma cells (part 1)

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H3K27me3 ChIP-seq in SH-SY5Y human neuroblastoma cell line

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