Transcriptomics

Dataset Information

0

Mitochondrial dsRNA triggers antiviral signalling in humans


ABSTRACT: Mitochondria are descendants of endosymbiotic bacteria and retain essential prokaryotic features such as a compact circular genome. Consequently, in mammals, mitochondrial DNA is subjected to bidirectional transcription that generates overlapping transcripts capable of forming long double-stranded RNA structures. However, to our knowledge, mitochondrial double-stranded RNA has not been previously characterized in vivo. Here, we describe the presence of a highly unstable native mitochondrial double-stranded RNA species at single cell level and identify keyroles for the degradosome components, mitochondrial dsRNA helicase SUV3 and polynucleotide phosphorylase PNPase in restricting the levels of mitochondrial double-stranded RNA. Loss of either enzyme results in massive accumulation of mitochondrial double-stranded RNA that escapes into the cytoplasm in a PNPase-dependent manner. This process engages an MDA5-driven antiviral signalling pathway that triggers a type I interferon response. Consistent with these data, patients carrying hypomorphic mutations in the gene PNPT1, which encodes PNPase, display mitochondrial double-stranded RNA accumulation coupled with upregulation of interferon-stimulated genes and other markers of immune activation. The localization of PNPase to the mitochondrial inter-membrane space and matrix suggests that it has a dual role in preventing the formation and release of mitochondrial double-stranded RNA into the cytoplasm. This in turn prevents the activation of potent innate immune defence mechanisms evolved to protect vertebrates against microbial and viral attack.

ORGANISM(S): Homo sapiens

PROVIDER: GSE94957 | GEO | 2018/05/03

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2018-07-06 | PXD009826 | Pride
2015-11-10 | GSE57784 | GEO
2023-07-07 | MSV000092370 | MassIVE
2021-10-26 | MODEL2110250001 | BioModels
2018-06-17 | GSE106368 | GEO
2018-05-03 | GSE109210 | GEO
2019-06-28 | GSE131742 | GEO
2019-04-01 | PXD012662 | Pride
| PRJNA416454 | ENA
2024-11-06 | PXD051180 | Pride