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The Drosophila Embryo at Single-Cell Transcriptome Resolution

ABSTRACT: By the onset of morphogenesis, Drosophila embryos consist of about 6000 cells that express distinct gene combinations. Here, we used single-cell sequencing of precisely staged embryos and devised DistMap, a computational mapping strategy to reconstruct the embryo and to predict spatial gene expression approaching single-cell resolution. We produce a virtual embryo with about 8000 expressed genes per cell. Our interactive “Drosophila-Virtual-Expression-eXplorer” (DVEX) database generates three-dimensional virtual in situ hybridizations and computes gene expression gradients. We used DVEX to uncover patterned expression of transcription factors and long noncoding RNAs, as well as signaling pathway components. Spatial regulation of Hippo signaling during early embryogenesis suggests a mechanism for establishing asynchronous cell proliferation. Our approach is suitable to generate transcriptomic blueprints for other complex tissues.

OTHER RELATED OMICS DATASETS IN: PRJNA375756

ORGANISM(S): Drosophila melanogaster Drosophila virilis

PROVIDER: GSE95025 | GEO | 2017/09/01

SECONDARY ACCESSION(S): PRJNA375756

REPOSITORIES: GEO

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