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BEARscc: robustness of single-cell clusters determined using simulated technical replicates

ABSTRACT: Technical variance is a major confounding factor in single-cell RNA sequencing, not least because measurements on the same cell are not replicable. We developed BEARscc, a tool that simulates experiment-specific technical replicates based on a probabilistic model of technical variance trained on RNA spike-in measurements. We demonstrate that the tool improves the unsupervised classification of cells and aids the interpretation of single-cell RNA-seq experiments.

OTHER RELATED OMICS DATASETS IN: PRJNA376200

ORGANISM(S): other sequences Homo sapiens

PROVIDER: GSE95155 | GEO | 2018/04/03

REPOSITORIES: GEO

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