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Transgenic Plasmodium falciparum blood stage parasites 3D7/GDV1-GFP-DD: control (-Shield-1;3D7/GDV1-GFP-DDOFF) vs GDV1-overexpressing (+Shield-1;3D7/GDV1-GFP-DDON) parasites

ABSTRACT: Transcriptional profiling of transgenic P. falciparum blood stage parasites expressing an ectopic copy of GDV1-GFP-DD (3D7/GDV1-GFP-DD). The DD (FKBP destabilisation domain) allows for the conditional expression of fusion proteins: DD fusion proteins are rapidly degraded or stably expressed in absence or presence of the stabilising ligand Shield-1, respectively (Banaszynski LA, Chen LC, Maynard-Smith LA, Ooi AG, Wandless TJ. A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules.Cell. 2006 Sep 8;126(5):995-1004). The goal of this experiment was to identify genes differentially expressed in response to induced GDV1 overexpression.

ORGANISM(S): Plasmodium falciparum 3D7 Plasmodium falciparum

PROVIDER: GSE95547 | GEO | 2017/03/02

SECONDARY ACCESSION(S): PRJNA377430

REPOSITORIES: GEO

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Transgenic Plasmodium falciparum blood stage parasites F12/GDV1-GFP-DD: control (-Shield-1;F12/GDV1-GFP-DDOFF) vs GDV1-overexpressing (+Shield-1;F12/GDV1-GFP-DDON) parasites

Project description:Transcriptional profiling of transgenic P. falciparum blood stage parasites expressing an ectopic copy of GDV1-GFP-DD (F12/GDV1-GFP-DD). The DD (FKBP destabilisation domain) allows for the conditional expression of fusion proteins: DD fusion proteins are rapidly degraded or stably expressed in absence or presence of the stabilising ligand Shield-1, respectively (Banaszynski LA, Chen LC, Maynard-Smith LA, Ooi AG, Wandless TJ. A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules.Cell. 2006 Sep 8;126(5):995-1004). The goal of this experiment was to identify genes differentially expressed in response to induced GDV1 overexpression.

2017-03-02 | GSE95548 | GEO

Transgenic Plasmodium falciparum parasites line 3D7/DDGFP-PfAP2-HC: transcriptional differences between 3D7/DDGFP-PfAP2-HC control parasites (cultured in presence of Shield-1; ON) vs PfAP2-HC-depleted parasites (cultured in absence of Shield-1; OFF)

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Plasmodium falciparum blood stage parasites: control vs PfTRF-depleted parasites

Project description:Transcriptional profiling of transgenic Plasmodium falciparum blood stage parasites expressing the telomere repeat-binding factor PfTRF as a C-terminal GFP-DD fusion (3D7/TRFGFP-ON). The DD (FKBP destabilisation domain) allows for the conditional depletion of fusion proteins in absence of the stabilising ligand Shield-1 (Banaszynski LA, Chen LC, Maynard-Smith LA, Ooi AG, Wandless TJ. A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules.Cell. 2006 Sep 8;126(5):995-1004). The goal of this experiment was to identify genes differentially expressed in response to PfTRF depletion.

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Transgenic Plasmodium falciparum blood stage parasites F12/GDV1-GFP-DD: control (-Shield-1 F12/GDV1-GFP-DDOFF) vs GDV1-overexpressing (+Shield-1 F12/GDV1-GFP-DDON) parasites

Project description:Transgenic Plasmodium falciparum blood stage parasites F12/GDV1-GFP-DD: control (-Shield-1 F12/GDV1-GFP-DDOFF) vs GDV1-overexpressing (+Shield-1 F12/GDV1-GFP-DDON) parasites

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GDV1 triggers sexual conversion and differentiation in malaria parasites by antagonizing HP1-dependent gene silencing

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Project description:Transgenic Plasmodium falciparum blood stage parasites 3D7/GDV1-GFP-DD: control (-Shield-1 3D7/GDV1-GFP-DDOFF) vs GDV1-overexpressing (+Shield-1 3D7/GDV1-GFP-DDON) parasites

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RNA-sequencing of parasites overexpressing conditionally stabilized BFD1 to identify genes regulated by BFD1

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Heterochromatin protein 1 secures survival and transmission of malaria parasites

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