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Wound regeneration deficit in rats correlates with low morphogenetic potential and distinct transcriptome profile of epidermis

ABSTRACT: We report whole tissue transcriptomes from rat and mouse wounds, as well as rat inter-follicular epidermis

OTHER RELATED OMICS DATASETS IN: PRJNA380583

ORGANISM(S): Mus musculus Rattus norvegicus

PROVIDER: GSE97047 | GEO | 2018/01/03

SECONDARY ACCESSION(S): PRJNA380583

REPOSITORIES: GEO

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Wound Regeneration Deficit in Rats Correlates with Low Morphogenetic Potential and Distinct Transcriptome Profile of Epidermis.

Project description:Large excisional wounds in mice prominently regenerate new hair follicles (HFs) and fat, yet humans are deficient for this regenerative behavior. Currently, wound-induced regeneration remains a clinically desirable, but only partially understood phenomenon. We show that large excisional wounds in rats across seven strains fail to regenerate new HFs. We compared wound transcriptomes between mice and rats at the time of scab detachment, which coincides with the onset of HF regeneration in mice. In both species, wound dermis and epidermis share core dermal and epidermal transcriptional programs, respectively, yet prominent interspecies differences exist. Compared with mice, rat epidermis expresses distinct transcriptional and epigenetic factors, markers of epidermal repair, hyperplasia, and inflammation, and lower levels of WNT signaling effectors and regulators. When recombined on the surface of excisional wounds with vibrissa dermal papillae, partial-thickness skin grafts containing distal pelage HF segments, but not interfollicular epidermis, readily regenerated new vibrissa-like HFs. Together, our findings establish rats as a nonregenerating rodent model for excisional wound healing and suggest that low epidermal competence and associated transcriptional profile may contribute to its regenerative deficiency. Future comparison between rat and mouse may lend further insight into the mechanism of wounding-induced regeneration and causes for its deficit.

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Wound regeneration deficit in rats correlates with low morphogenetic potential and distinct transcriptome profile of epidermis

Project description:Wound regeneration deficit in rats correlates with low morphogenetic potential and distinct transcriptome profile of epidermis

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Wound epidermis-dependent transcriptional programs

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Project description:Using microarray analysis, we explored the differences in gene expression in wounded and intact skin using murine model. Injured skin samples were examined at days 1 and 4 post injury. The results provide the detailed molecular profile of the the genetic response to injury. Two full-thickness dermal wounds were made on the opposite sides of the midline of each mouse using a 4 mm punch biopsy instrument. Wounds were made through the epidermis, dermis, and subcutaneous tissue layers while leaving the fascia intact. At a specified time point after the wounding (1 and 4 days), mice were sacrificed by carbon dioxide inhalation. The wounds and surrounding tissues or intact skin samples were removed with an 8 mm biopsy punch.

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Connective tissue regeneration dynamics persistently alter epithelial dynein DNAH10 expression - DNAH10 overexpression in psoriatic lesional epidermis

Project description:The objective of this study was to investigate how active dermal connective tissue composition dynamics affect long term protein expression in the epidermis. After surgically removing damaged skin to its full thickness at the site of a deep burn in ten patients, we randomized three adjacent test areas within the wounds to receive either: 1) no dermal component as control, 2) a permanent artificial dermal matrix or 3) a temporary dressing to induce formation of granulation tissue. Each area was then covered with an autologous superficial healty skin transplant as the epidermal indicator. One year later, biopsy samples were taken from each site. The epidermis and dermis were selectively microdissected by laser-capture from cut tissue biopsy sections, and analyzed using untargeted non-labelled quantitative proteomics.

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Defining stem cell dynamics and migration during wound healing in mouse skin epidermis (D4 post-wound)

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Project description:Human in vivo skin wound: Non-wounded skin was obtained by taking punch biopsies from three healthy donors (donor 1,2 and 3). The samples were termed 'skin day 0 in vivo wound'. Skin wound samples were retrieved by making new punch biopsies from the edge of the original biopsies after four days. These samples were termed 'skin day 4 in vivo wound'. As much dermal tissue as possible was removed by dissection to make sure mainly epidermis was present in the samples. The samples were washed in NaCl to possible remove infiltrating inflammatory cells before RNA isolation. Ex vivo skin wounds: Skin was obtained from three healthy donors following reduction surgery (donor 1, 2, and 3). As much dermal tissue as possible was removed dissection. These samples were termed 'skin day 0 ex vivo wound'. Skin was sliced into 1x10 mm slices and incubated in keratinocyte medium for four days with either 1:1000 fold dilution of DMSO or 10 micromolar AG-1478 (dissolved in DMSO). Again as much dermal tissue was removed by dissection as possible before RNA was isolated. These samples were termed 'skin day 4 ex vivo wound' and 'skin day 4 AG-1478 ex vivo wound'. By comparing the gene expression day 4 in ex vivo wound with in vivo wounds it was possible to see which part of the gene expression in wounded skin that was due to the epidermal reaction to injury and how much was due to stimuli from infiltrating inflammatory cells absent in the ex vivo skin wounds. By comparing the data from ex vivo skin wounds day 4 with and without the EGFR-inhibitor AG-1478, it was possible to look at the importance of the EGF-receptor of EGFR for the gene expression in ex vivo wounded skin.

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Transcriptional programming of human epidermis during wound repair and in pressure ulcer at single-cell resolution

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2021-03-28 | GSE137897 | GEO

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