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Wound regeneration deficit in rats correlates with low morphogenetic potential and distinct transcriptome profile of epidermis

ABSTRACT: Wound regeneration deficit in rats correlates with low morphogenetic potential and distinct transcriptome profile of epidermis

OTHER RELATED OMICS DATASETS IN: GSE97047

PROVIDER: PRJNA380583 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5378800.fastq.gz	Fastqsanger.gz
	SRR5378810.fastq.gz	Fastqsanger.gz
	SRR5378801.fastq.gz	Fastqsanger.gz
	SRR5378811.fastq.gz	Fastqsanger.gz
	SRR5378802.fastq.gz	Fastqsanger.gz

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Wound regeneration deficit in rats correlates with low morphogenetic potential and distinct transcriptome profile of epidermis

Project description:We report whole tissue transcriptomes from rat and mouse wounds, as well as rat inter-follicular epidermis

2018-01-03 | GSE97047 | GEO

Wound epidermis-dependent transcriptional programs

Project description:The wound epidermis is required for limb regeneration. To elucidate the roles of the wound epidermis during early regeneration, we examined how the transcriptional programs of early dividing cells (enriched for blastemal progenitors) and non-dividing cells in regenerating stump tissues as well as epithelial cells change when we prevented wound epidermis formation.

2020-01-15 | GSE132317 | GEO

Wound epidermis-dependent transcriptional programs

Project description:Wound epidermis-dependent transcriptional programs

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Wound Regeneration Deficit in Rats Correlates with Low Morphogenetic Potential and Distinct Transcriptome Profile of Epidermis.

Project description:Large excisional wounds in mice prominently regenerate new hair follicles (HFs) and fat, yet humans are deficient for this regenerative behavior. Currently, wound-induced regeneration remains a clinically desirable, but only partially understood phenomenon. We show that large excisional wounds in rats across seven strains fail to regenerate new HFs. We compared wound transcriptomes between mice and rats at the time of scab detachment, which coincides with the onset of HF regeneration in mice. In both species, wound dermis and epidermis share core dermal and epidermal transcriptional programs, respectively, yet prominent interspecies differences exist. Compared with mice, rat epidermis expresses distinct transcriptional and epigenetic factors, markers of epidermal repair, hyperplasia, and inflammation, and lower levels of WNT signaling effectors and regulators. When recombined on the surface of excisional wounds with vibrissa dermal papillae, partial-thickness skin grafts containing distal pelage HF segments, but not interfollicular epidermis, readily regenerated new vibrissa-like HFs. Together, our findings establish rats as a nonregenerating rodent model for excisional wound healing and suggest that low epidermal competence and associated transcriptional profile may contribute to its regenerative deficiency. Future comparison between rat and mouse may lend further insight into the mechanism of wounding-induced regeneration and causes for its deficit.

| S-EPMC6059613 | biostudies-literature

Defining stem cell dynamics and migration during wound healing in mouse skin epidermis (D4 post-wound)

Project description:Wound healing is essential to repair the skin after injury. In the epidermis, distinct stem cells (SCs) populations contribute to wound healing. However, how SCs balance proliferation, differentiation and migration to repair a wound remains poorly understood. Here we show the cellular and molecular mechanisms that regulate wound healing in mouse tail epidermis. Using a combination of proliferation kinetics experiments and molecular profiling, we identify the gene signatures associated with proliferation, differentiation and migration in different regions surrounding the wound. Functional experiments show that SC proliferation, migration and differentiation can be uncoupled during wound healing. Lineage tracing and quantitative clonal analysis reveal that, following wounding, progenitors divide more rapidly, but conserve their homeostatic mode of division, leading to their rapid depletion whereas SCs become active, giving rise to new progenitors that expand and repair the wound. These results have important implications for tissue regeneration, acute and chronic wound disorders.

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Defining stem cell dynamics and migration during wound healing in mouse skin epidermis

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Global microarray analysis of changes of gene expression in the epidermis of K14-caSmad1 mice

Project description:Bone morphogenetic protein (BMP) signalling plays a key role in the control of skin development and postnatal remodelling by regulating keratinocyte proliferation, differentiation and apoptosis. To study the role of BMPs in wound-induced epidermal repair, we used transgenic mice overexpressing the BMP downstream component Smad1 under the control of a K14 promoter as an in vivo model, as well as ex vivo and in vitro assays. K14-caSmad1 mice exhibited retarded wound healing associated with significant inhibition of proliferation and increased apoptosis in healing wound epithelium. Furthermore, microarray and qRT-PCR analyses revealed decreased expression of a number of cytoskeletal/cell motility-associated genes including wound-associated keratins (Krt16, Krt17) and Myo5a, in the epidermis of K14- caSmad1 mice versus wild-type controls during wound healing. BMP treatment significantly inhibited keratinocyte migration ex vivo, and primary keratinocytes of K14-caSmad1 mice showed retarded migration compared to wild-type controls. Finally, siRNA-mediated silencing of Bmpr-1B in primary mouse keratinocytes accelerated cell migration and was associated with increased expression of Krt16, Krt17 and Myo5a compared to controls. Thus, this study demonstrates that BMPs inhibit keratinocyte proliferation, cytoskeletal organization and migration in regenerating skin epithelium during wound healing, and raises a possibility for using BMP antagonists for the management of chronic wounds. Two-condition experiment, Wild type vs. Smad1 overexpressing mice. Biological replicates: 2 replicates.

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2020-04-02 | PXD010837 | Pride

Impaired wound healing in Snhg26 knockout mice

Project description:The wounds were made on the back skin of Snhg26 knockout (KO) and wild type (WT) mice. The wound edge and skin tissue were collected and the epidermis were separated by incubate the tissue with dispaseII. Total RNA was extracted from the epidermis. The global transcriptome analysis of the epidermis were performed by using Affymetrix arrays.

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Global microarray analysis of changes of gene expression in the epidermis of K14-caSmad1 mice

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