Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Molecular transitions in early progenitors during human cord blood hematopoiesis

ABSTRACT: Hematopoietic stem cells give rise to diverse cell types in the blood system, yet our molecular understanding of the earliest fate decisions that generate this enormous diversity in humans remains incomplete. Here we leverage Drop-seq to individually profile more than 20,000 progenitors cells from human cord blood, without prior enrichment or depletion for individual lineages based on surface markers. Our data reveal a transcriptional 'atlas' of progenitor states in human cord blood, which we leverage to reconstruct differentiation trajectories from HSC to four downstream lineages. And we also demonstrate that Drop-seq data can be utilized to identify new heterogeneous markers of cell state.

ORGANISM(S): Homo sapiens

PROVIDER: GSE97104 | GEO | 2018/03/15

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Isolation and comparative analysis of mesenchymal stem cells from human umbilical cord

Project description:A non-controversial and non-invasive source of adult stem cells (ASCs), particularly hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs) is human umbilical cord blood. HSCs derived from cord blood have been used for treating leukemia and other blood disorders for the last 30 years. While the presence of MSCs in cord blood is limited, umbilical cord has been found to be promising source of MSCs. However, the cord is an anatomically complex organ and potential isolation of MSCs from its various parts has not been fully explored. In this study we dissected the cord into cord placenta junction (CPJ), cord tissue (CT), and Wharton’s jelly (WJ) and isolated stem cells. These cells exhibited fibroid morphology, expressed MSC-specific markers including CD90, CD73, CD105, CD44, and CD29 and differentiated into chondrogenic, osteogenic, myogenic and neurogenic lineages. In addition, they all expressed pluripotency genes, OCT4, Nanog, Sox2 and KLF4 but expression of these markers was highest in CPJ followed by WJ and CT. CPJ-MSCs also had higher rate of proliferation compared to WJ- and CT-MSCs. Proliferation of WJ- and CT-MSCs was markedly decreased upon passaging with concomitant decrease in expression of MSC and pluripotency markers. Based on their greater self-renewal potential, CPJ-MSCs could be superior to WJ- and CT-MSCs for the applications in therapeutic and regenerative medicine.

2016-06-08 | GSE72597 | GEO

Isolation and comparative analysis of mesenchymal stem cells from human umbilical cord II

Project description:A non-controversial and non-invasive source of adult stem cells (ASCs), particularly hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs) is human umbilical cord blood. HSCs derived from cord blood have been used for treating leukemia and other blood disorders for the last 30 years. While the presence of MSCs in cord blood is limited, umbilical cord has been found to be promising source of MSCs. However, the cord is an anatomically complex organ and potential isolation of MSCs from its various parts has not been fully explored. In this study we dissected the cord into cord placenta junction (CPJ), cord tissue (CT), and Wharton’s jelly (WJ) and isolated stem cells. These cells exhibited fibroid morphology, expressed MSC-specific markers including CD90, CD73, CD105, CD44, and CD29 and differentiated into chondrogenic, osteogenic, and adipogenic lineages. In addition, they all expressed pluripotency genes, OCT4, Nanog, Sox2 and KLF4 but expression of these markers was highest in CPJ followed by WJ and CT. CPJ-MSCs also had higher rate of proliferation compared to WJ- and CT-MSCs. Proliferation of WJ- and CT-MSCs was markedly decreased upon passaging with concomitant decrease in expression of MSC and pluripotency markers. Based on their greater self-renewal potential, CPJ-MSCs could be superior to WJ- and CT-MSCs for the applications in therapeutic and regenerative medicine.

2016-06-08 | GSE76295 | GEO

Single cell transcriptomics of index-sorted dendritic cell precursors from human peripheral blood

Project description:The transcriptomes of single cells from human perpheral blood were investigated at single cell level, with the aim to reconstruct the development of human dendritic cell (DC) lineages.

2020-08-01 | GSE143158 | GEO

Transcriptomic profiling of 27-year cryopreserved umbilical cord blood HSCs/HPCs

Project description:Umbilical cord blood banking is critical for the success of umbilical cord blood transplants. Here we analyzed transcriptomic differences between 27-year cryopreserved umbilical cord blood hematopoietic stem cells (HSCs) and multipotent progenitor cells (MPPs) and those derived from fresh cord blood. We also leveraged differences in engraftment capacity to examine the transcriptomes of HSCs/HPCs defined by engraftment capacity, demonstrating the feasibility of this approach for identifying potency markers to aid in the selection of cord blood units for transplantation and revealing novel potential regulators of cord blood HSC/HPC engraftment.

2023-10-04 | GSE225699 | GEO

BLUEPRINT ChIP-seq data for cells in the haematopoietic lineages, from adult and cord blood samples.

Project description:ChIP-seq data for cells in the haematopoietic lineages, from adult and cord blood samples.

| EGAS00001000326 | EGA

BLUEPRINT RNA-seq data for common cells in the haematopoietic lineages, from adult and cord blood samples.

Project description:RNA-seq data for common cells in the haematopoietic lineages, from adult and cord blood samples.

| EGAS00001000327 | EGA

BLUEPRINT ATAC-seq data for cells in the haematopoietic lineages, from adult and cord blood samples

Project description:BLLUEPRINT ATAC-seq data for cells in the haematopoietic lineages, from adult and cord blood samples.

| EGAS00001001596 | EGA

BLUEPRINT RNA-seq data for rare cells in the haematopoietic lineages, from adult and cord blood samples.

Project description:RNA-seq data for rare cells in the haematopoietic lineages, from adult and cord blood samples.

| EGAS00001000284 | EGA

KSRP specifies monocytic and granulocytic differentiation through regulating miR-129 biogenesis and RUNX1 expression

Project description:We used CD34-positive cells isolated from cord blood to differentiate to granulocytic and monocytic lineages, respectively. Poly A-enriched RNA-seq was performed on the day 5, 10 and 15 samples of both granulocytic and monocytic lineages.

2017-08-01 | GSE87088 | GEO

Immunel Landscape of Spinal Cord after Injury in Mice Using Single-Cell RNA-Seq

Project description:Single-cell RNA sequencing of immune cells of mouse spinal cord using Drop-Seq

2021-08-28 | GSE182803 | GEO