Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis of normal human lung fibroblasts (NHLFs) following TBX4 knockdown.

ABSTRACT: TBX4 is a transcription factor unique to lung fibroblasts and is associated with super-enhancer. RNA-sequencing analysis on NHLFs following TBX4 knockdown revealed a broad array of genes possibly regulated by TBX4.

ORGANISM(S): Homo sapiens

PROVIDER: GSE97266 | GEO | 2017/12/20

SECONDARY ACCESSION(S): PRJNA381149

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptome analysis of normal human lung fibroblasts (NHLFs) following FOXL1 knockdown.

Project description:FOXL1 is a key transcription factor that characterizes the pulmonary origin of lung fibroblasts.. RNA-sequencing analysis on NHLFs following FOXL1 knockdown revealed a broad array of genes possibly regulated by FOXL1.

2020-10-07 | GSE137823 | GEO

Identification and function of Tbx4 resident fibroblasts as a major source of fibrotic fibroblasts

Project description:Progressive tissue fibrosis is a major cause of morbidity, and idiopathic pulmonary fibrosis (IPF) is a terminal illness characterized by unremitting matrix deposition in the lung with very limited choice of therapies. The imcomplete understanding of the mechanisms of progressive fibrosis curbs the progress in therapeutics development. Of which, the origin of fibrotic fibroblasts has been poorly defined during the pathogenesis of tissue fibrosis. Here, we fate-mapped a early embryonic transcription factor T-box gene 4 (Tbx4)-derived mesenchymal progenitors in injured adult lung and found that Tbx4+ lineage cells are the major source of myofibroblasts. The ablation of Tbx4+ cells or disruption of Tbx4 signaling attenuated lung fibrosis in bleomycin injury model in mice in vivo. Furthermore, Tbx4+ fibroblasts are more invasive and the regulation of fibroblast invasiveness by Tbx4 is through mediating hyaluronan synthase 2 (HAS2). This study identified a major mesenchymal transcription factor driving the development of fibrotic fibroblasts during lung fibrosis. Understanding the origin, signaling, and functions of these fibroblasts would prove pivotal in the development of therapeutics for patients with progressive fibrotic diseases. We used microarrays to detail the gene expression of Tbx4 and non-Tbx4 cultured fibroblasts.

2017-08-23 | GSE68427 | GEO

Transcriptome analysis of normal human lung fibroblasts (NHLFs) following FOXL1 knockdown.

Project description:Transcriptome analysis of normal human lung fibroblasts (NHLFs) following FOXL1 knockdown.

| PRJNA573534 | ENA

Fibroblasts: Normal fibroblasts vs. Carcinoma-associated fibroblasts

Project description:Fibroblasts: Normal fibroblasts vs. Carcinoma-associated fibroblasts

| PRJNA690801 | ENA

RNA-seq analysis following REVERBalpha knockdown in fibroblasts

Project description:RNA-seq analysis following REVERBalpha knockdown in fibroblasts

| PRJEB35253 | ENA

RNA-seq analysis following REVERBalpha knockdown in fibroblasts

Project description:The aim of the study was to identify how REVERBalpha modulates myofibroblast differentiation; by sequencing fibroblasts twelve and twenty-four hours after siRNA knockdown.

2019-12-10 | E-MTAB-8499 | biostudies-arrayexpress

Expression data from human fetal lung normal diploid fibroblasts

Project description:The interaction between cancer and stroma plays a key role in tumor progression. Inactivation of p53 is often observed in stromal cells surrounding in cancer, suggesting that p53 in fibroblasts is involved in tumor progression. To elucidate the mechanism by which p53 loss in fibroblasts effect on proliferation and invasiveness of cancer cells, we performed comprehensive expression profiling analyses between p53 knockdown and control fibroblasts using GeneChip Human Genome U133 plus 2.0 arrays.

2014-08-01 | GSE58753 | GEO

Expression data from (mouse) normal lung fibroblasts and carcinoma-associated fibroblasts

Project description:Cancer-associated fibroblasts (CAFs) have been reported to support tumor progression by a variety of mechanisms. However, their role in the progression of non-small cell lung cancer (NSCLC) remains poorly defined. In addition, the extent to which specific proteins secreted by CAFs contribute directly to tumor growth is unclear. To study the role of CAFs in NSCLC, a cross-species functional characterization of mouse and human lung CAFs was performed, including gene expression analysis comparing normal mouse lung fibroblasts (NFs) and mouse lung CAFs to seek for differentially-expressed secreted proteins. Gene expression microarrays were used to identify transcriptomic changes between NFs and CAFs that may contribute to their different tumor-enhancing capacity.

2013-07-10 | GSE48397 | GEO

Expression data from human fetal lung normal diploid fibroblasts

2014-08-01 | E-GEOD-58753 | biostudies-arrayexpress

Ubiquitin Proteomics Analysis of Systemic Sclerosis Lung Fibroblasts with or without KLHL42 knockdown

Project description:Systemic scleroderma (SSc) is an autoimmune disease which results in fibrotic production in the lung. Resultant SSC-pulmonary fibrosis is the main cause of mortality among SSc patients. From high throughput RNAi screening, we uncovered the ubiquitin E3 ligase KLHL42 as a potential pro-fibrotic mediator of TGFb-dependent fibrotic signaling in primary SSc lung fibroblasts. In this analysis, we sought to uncover putative substrates for KLHL42 by comparing SSc lung fibroblasts with control or KLHL42 siRNA prior to TGFb-treatment, lysis, and TUBE precipitation. The resultant pull-down was analyzed with LC-MS/MS.

2020-01-14 | MSV000084800 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data