Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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CRISPR-MIP replaces PCR and reveals GC and oversampling bias in pooled CRISPR screens


ABSTRACT: This is data for the evaluation of a new way of counting sgRNAs in CRISPR screens using padlock probes and UMIs. It is compared to the typical PCR-based approach. In particular, a dropout screen was performed in MiaPaCa-2 cells using the Human Kinome CRISPR pooled library (Addgene #75314)

INSTRUMENT(S): NextSeq 550

ORGANISM(S): synthetic construct

SUBMITTER: Johan Henriksson 

PROVIDER: E-MTAB-14379 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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