CRISPR-MIP replaces PCR and reveals GC and oversampling bias in pooled CRISPR screens
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ABSTRACT: This is data for the evaluation of a new way of counting sgRNAs in CRISPR screens using padlock probes and UMIs. It is compared to the typical PCR-based approach. In particular, a dropout screen was performed in MiaPaCa-2 cells using the Human Kinome CRISPR pooled library (Addgene #75314)
INSTRUMENT(S): NextSeq 550
ORGANISM(S): synthetic construct
SUBMITTER: Johan Henriksson
PROVIDER: E-MTAB-14379 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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