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The novel heme-dependent inducible protein, SRRD regulates heme biosynthesis and circadian rhythms

ABSTRACT: In order to investigate the function of heme in the regulation of gene expression, we herein examined variations in mRNA levels in ALA-treated cells from control conditions. A comprehensive anal- ysis by RNA sequencing showed marked changes in the expression of various genes. Among the different amounts of mRNA, we identified the novel heme-inducible protein, SRRD. The plant ho- mologue Sensitivity to Red Light Reduced (SRR1) was previously reported to be involved in the regulation of the circadian clock and phytochrome B signaling in Arabidopsis thaliana. We found that SRRD regulated not only heme biosynthesis, but also the expression of clock genes. The involvement of SRRD in the prolif- eration of cells was also demonstrated.

ORGANISM(S): Mus musculus

PROVIDER: GSE98158 | GEO | 2017/11/02

SECONDARY ACCESSION(S): PRJNA384175

REPOSITORIES: GEO

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GATA-1 and heme regulate the erythroid cell transcriptome.

Project description:Alas2 gene encodes the rate-limiting enzyme in heme biosynthesis. CRISPR/Cas9-mediated ablation of two Alas2 intronic cis-elements strongly reduced GATA-1-induced Alas2 transcription, heme biosynthesis, and GATA-1 regulation of other vital constituents of the erythroid cell transcriptome. Bypassing Alas2 function in Alas2 cis-element-mutant (double mutant) cells by providing its catalytic product 5-aminolevulinic acid (5-ALA) rescued heme biosynthesis and the GATA-1-dependent genetic network. We discovered a GATA factor- and heme-dependent circuit that establishes the erythroid cell transcriptome. G1E-ER-GATA-1 WT and double mutant cells were examined. Untreated WT, beta-estradiol-treated WT, beta-estradiol-treated double-mutant, and beta-estradiol/5-ALA-treated double-mutant cells were subjected to RNA-seq.

2016-01-07 | E-GEOD-74371 | biostudies-arrayexpress

Co-administration of angiotensin II and simvastatin triggers kidney injury upon heme oxygenase-1 deficiency

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GATA-1 and heme regulate the erythroid cell transcriptome.

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Identification of Cytoprotective Small Molecule Inducers of Heme Oxygenase-1

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Heme oxygenase-1 orchestrates the immunosuppressive program of tumor-associated macrophages [ATAC-Seq]

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Transcription profiling of S. cerevisiae response to heme deficiency and hypoxia

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2008-06-16 | E-GEOD-9514 | biostudies-arrayexpress

ATAC-seq reveals GATA-1- and heme-dependent chromatin targeting in erythroid cells.

Project description:CRISPR/Cas9-mediated ablation of two Alas2 intronic cis-elements in G1E-ER-GATA1 cells strongly reduced GATA-1-induced Alas2 transcription, heme biosynthesis, and GATA-1 regulation of other vital constituents of the erythroid cell transcriptome. Bypassing Alas2 function in Alas2 cis-element-mutant (double mutant) cells by providing its catalytic product 5-aminolevulinic acid (5-ALA) rescued heme biosynthesis and a subset of GATA-1-dependent genetic network. Using the same system, we discovered a GATA-1- and heme-dependent circuit that regulates chromatin accessibility during erythroid maturation.

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