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Wild type vs glnPA double mutant

ABSTRACT: This microarray experiment is part of a study addressing the importance of glutamine metabolism in Streptococcus pneumoniae for the virulence of this bacterium. To be able to gain more insight into the phenotype of a double mutant of glnA (TIGR 4 locus tag SP0502, encoding glutamine synthetase) and glnP (SP1241, encoding a permease component of a glutmine uptake ABC transporter), the transcriptome of this mutant, the glnPA double mutant, was determined in strain S. pneumoniae D39 grown in rich GM17 medium with 0.5 mg/ml glutamine. This revealed a big change in transcriptome in the mutant, especially a lot of amino acid and peptide metabolic genes. Keywords: transcriptome analysis of D39 wild-type compared with its isogenic glnPA double mutant

ORGANISM(S): Streptococcus pneumoniae

PROVIDER: GSE9850 | GEO | 2008/02/01

SECONDARY ACCESSION(S): PRJNA103825

REPOSITORIES: GEO

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Wild type vs glnPA double mutant

Project description:This microarray experiment is part of a study addressing the importance of glutamine metabolism in Streptococcus pneumoniae for the virulence of this bacterium. To be able to gain more insight into the phenotype of a double mutant of glnA (TIGR 4 locus tag SP0502, encoding glutamine synthetase) and glnP (SP1241, encoding a permease component of a glutmine uptake ABC transporter), the transcriptome of this mutant, the glnPA double mutant, was determined in strain S. pneumoniae D39 grown in rich GM17 medium with 0.5 mg/ml glutamine. This revealed a big change in transcriptome in the mutant, especially a lot of amino acid and peptide metabolic genes. Keywords: transcriptome analysis of D39 wild-type compared with its isogenic glnPA double mutant The wild-type strain D39 and its isogenic glnPA double mutant were grown in 4 biological replicates in GM17 with 0.5 mg/ml glutamine to an Optical Density at 600 nm of 0.3. From these cultures samples were prepared for transciptome determination with in-house printed microarrays covering the genomes of several S. pneumoniae species. The wild-type (control, Ch1) was labeled with Cy5 (3 samples) or Cy3 (1 sample) and vice versa for the samples of the glnPA mutant (target, Ch2). Samples were pair-wise compared on glass-microarray slides.

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Project description:This study addresses the response of Streptococcus pneumoniae to zinc. The wild-type strain D39 was grown in GM17 and GM17 with 0.25 mM of ZnSO4. The transcriptome under these conditions was compared by using microarrays. Keywords: transcriptome analysis of D39 wild-type compared with its isogenic glnPA double mutant The wild-type strain D39 was grown in 4 biological replicates in GM17 and in GM17+0.25 mM ZnSO4 to an Optical Density at 600 nm of 0.3. From these cultures samples were prepared for transciptome determination with in-house printed microarrays covering the genomes of several S. pneumoniae species. The GM17 samples (CH1) and GM17+ZnSO4 samples (CH2) were labeled with Cy5 or Cy3. Samples were hybridized to glass-microarray slides. This work is part of a publication that is likely to be released in june/july 2008: 'Opposite effects of Mn2+ and Zn2+ on the PsaR-mediated expression of the virulence genes pcpA, prtA and psaBCA of Streptococcus pneumoniae" by Kloosterman et al.

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Impact of bguR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Glucose

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 ΔbguR to D39 wild-type grown in M17 medium + 0.5% glucose (GM17).

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Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 ΔrokA to D39 wild-type grown in M17 medium + 0.5% glucose (GM17).

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