Phagocytic receptor type affects phagosome maturation, gene expression and phagosome protein composition in macrophages

ABSTRACT: The receptors engaged during phagocytic particle uptake determine the signalling events that occur during phagosome formation and maturation.However, pathogens generally have multiple ligands, making it difficult to dissect the roles of individual receptors in these processes. Here,we used latex beads coupled to single ligands, focusing primarily on IgG, mannan and avidin, and for some experiments on LPS, and monitored 1. phagocytic uptake rates, 2. fusion with lysosomal compartments, 3.macrophage gene expression 4. proteomic composition of isolated phagosomes and 5. the binding of phagosomes to actin filaments. Phagosomes formed in response to mannan fused with lysosomes earlier after uptake than the ones formed by the other ligands. The pattern of gene expression and the protein composition of isolated phagosomes showed that each bead ligand upregulated a distinct pattern of genes and led to a different composition of phagosomes. Furthermore, isolated phagosomes also exhibited differences in binding to F-actin in vitro.These data argue that activation of each receptor initiates a specific signature of signalling events that last many hours and influences several phagocytosis functions. Keywords: receptor-ligand interactions

ORGANISM(S): Mus musculus

PROVIDER: GSE9859 | GEO | 2007/12/12

SECONDARY ACCESSION(S): PRJNA103841

REPOSITORIES: GEO